The Kaposi's-sarcoma-associated herpesvirus orf35 gene product is required for efficient lytic virus reactivation

Shir Bergson, Inbal Itzhak, Talya Wasserman, Anastasia Gelgor, Inna Kalt, Ronit Sarid

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) is implicated in the etiology of several human malignancies. KSHV open reading frame (orf) 35 encodes a conserved gammaherpesvirus protein with an, as yet, unknown function. Employing the bacterial artificial chromosome (BAC) system, we generated a recombinant viral clone that fails to express ORF35 (BAC16-ORF35-stop) but preserves intact adjacent and overlapping reading frames. Using this construct, we studied the role of this previously uncharacterized gene product during lytic reactivation of KSHV. Upon lytic reactivation, the ORF35-stop recombinant virus displayed significantly reduced lytic viral gene expression, viral DNA replication, and progeny virus production as compared to control wild-type virus. Exogenous expression of ORF35-Flag reversed the effects of ORF35 deficiency. These results demonstrate that ORF35 is important for efficient lytic virus reactivation.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalVirology
Volume499
DOIs
StatePublished - 1 Dec 2016

Bibliographical note

Publisher Copyright:
© 2016

Keywords

  • BAC16
  • Kaposi's sarcoma-associated herpesvirus, KSHV
  • Lytic infection
  • Open reading frame 35 (ORF35)
  • Reactivation

Fingerprint

Dive into the research topics of 'The Kaposi's-sarcoma-associated herpesvirus orf35 gene product is required for efficient lytic virus reactivation'. Together they form a unique fingerprint.

Cite this