The interaction between β-carotene and lipoxygenase in plant and animal systems

Liat Lomnitski; Ronit Bar-Natan; David Sklan; Shlomo Grossman

doi:10.1016/0005-2760(93)90237-4

The interaction between β-carotene and lipoxygenase in plant and animal systems

Liat Lomnitski, Ronit Bar-Natan, David Sklan, Shlomo Grossman

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The effect of β-carotene (BC) on the activity of lipoxygenase (LOX) from plant and animal sources has been examined. Soybean lipoxygenase L-2 activity towards linoleate was inhibited by BC by a maximum of 70% at pH 6.5, whereas L-1 activity was little affected at pH 9.0. Lineweaver-Burk plots indicated that BC inhibited LOX activity by mixed competitive/non-competitive mechanisms. Other hydrophobic compounds also inhibited LOX activity; oleic acid and retinol were competitive inhibitors whereas tocopherol acetate and 5,8,11,14-eicosatetraynoic acid (ETYA) were non-competitive inhibitors. Binding studies with L-2 LOX bound to Sepharose indicated BC-binding and inhibition with the immobilized LOX. Activity of LOX from animal sources was also inhibited by BC both towards linoleate and arachidonate.

Original language	English
Pages (from-to)	331-338
Number of pages	8
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	1167
Issue number	3
DOIs	https://doi.org/10.1016/0005-2760(93)90237-4
State	Published - 23 Apr 1993

Keywords

Inhibition
Lipoxygenase
Peroxidation
β-Carotene

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10.1016/0005-2760(93)90237-4

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abstract = "The effect of β-carotene (BC) on the activity of lipoxygenase (LOX) from plant and animal sources has been examined. Soybean lipoxygenase L-2 activity towards linoleate was inhibited by BC by a maximum of 70% at pH 6.5, whereas L-1 activity was little affected at pH 9.0. Lineweaver-Burk plots indicated that BC inhibited LOX activity by mixed competitive/non-competitive mechanisms. Other hydrophobic compounds also inhibited LOX activity; oleic acid and retinol were competitive inhibitors whereas tocopherol acetate and 5,8,11,14-eicosatetraynoic acid (ETYA) were non-competitive inhibitors. Binding studies with L-2 LOX bound to Sepharose indicated BC-binding and inhibition with the immobilized LOX. Activity of LOX from animal sources was also inhibited by BC both towards linoleate and arachidonate.",

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AU - Sklan, David

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AB - The effect of β-carotene (BC) on the activity of lipoxygenase (LOX) from plant and animal sources has been examined. Soybean lipoxygenase L-2 activity towards linoleate was inhibited by BC by a maximum of 70% at pH 6.5, whereas L-1 activity was little affected at pH 9.0. Lineweaver-Burk plots indicated that BC inhibited LOX activity by mixed competitive/non-competitive mechanisms. Other hydrophobic compounds also inhibited LOX activity; oleic acid and retinol were competitive inhibitors whereas tocopherol acetate and 5,8,11,14-eicosatetraynoic acid (ETYA) were non-competitive inhibitors. Binding studies with L-2 LOX bound to Sepharose indicated BC-binding and inhibition with the immobilized LOX. Activity of LOX from animal sources was also inhibited by BC both towards linoleate and arachidonate.

KW - Inhibition

KW - Lipoxygenase

KW - Peroxidation

KW - β-Carotene

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The interaction between β-carotene and lipoxygenase in plant and animal systems

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Keywords

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