The influence of temperature on photodynamic cell killing in vitro with 5-aminolevulinic acid

Asta Juzeniene, Petras Juzenas, Irena Bronshtein, Alexander Vorobey, Johan Moan

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Cell survival was investigated after exposing cells in vitro to different temperatures before or after photodynamic therapy with 5-aminolevulinic acid. The photodynamic process was found to be temperature dependent. Cells exposed for 1 h to 41 °C before light exposure or to 7 °C after light exposure showed decreased survival. Furthermore, the photobleaching rate of protoporphyrin IX in the cells was found to increase with increasing temperature during the light exposure. Thus, the photodynamic effect with 5-aminolevulinic acid may be enhanced by heating the tumour area before, and by cooling it immediately after the treatment.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalJournal of Photochemistry and Photobiology B: Biology
Volume84
Issue number2
DOIs
StatePublished - 1 Aug 2006

Bibliographical note

Funding Information:
This work was supported by the Research Foundation of the Norwegian Radium Hospital (RF) and by the Norwegian Cancer Society (Kreftforeningen).

Keywords

  • 5-Aminolevulinic acid
  • Photodynamic therapy
  • Protoporphyrin IX

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