In this study we describe the cloning of a human gene, encoding a protein that shares 90% identity and 93% similarity at the primary structure level, with the mouse Pim-2 gene. The gene was designated hPim-2. Structural features suggest that like the mouse Pim-2, hPim-2 is also a serine threonine kinase. At the RNA level, two hPim-2 transcripts were identified. The first, 2.2 kb, is highly expressed in hematopoietic tissues and in leukemic and lymphoma cell lines (K-562, HL-60 and RAJI). It also shows considerable high levels in testis, small intestine, colon and human colorectal adenocarcinoma cells (SW480). A second transcript, 5.0 kb in size, could be detected only in spleen, thymus, small intestine and colon and in the K-562 and RAJI cell lines. In situ hybridization analysis of biopsies taken from testes of men with complete or partial spermatogenesis revealed that the gene is expressed in primary spermatocytes. In the absence of germ cells, signal could be detected over specific cells in the well developed interstitial region. These results suggest a role for hPim-2 in proliferating cells as well as during meiosis. A possible connection between hPim-2 and apoptosis is discussed.
|Number of pages||12|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|State||Published - 8 Nov 1998|
Bibliographical noteFunding Information:
The authors are grateful to Prof. Samuel Salzberg, Dr. Ron Wides, Mrs. Mira Malkov and Mrs. Rachel Steiner for reviewing the manuscript and for their critical comments. This work was supported in part by a grant from the Israel Cancer Association, the Israel Science Foundation administered by the Israel Academy of Sciences and Humanities, a grant from the Bar-Ilan committee for the promotion of research and the Jaimes Lucinchi Fund.