TY - JOUR
T1 - The generation of hybrid electrospun nanofiber layer with extracellular matrix derived from human pluripotent stem cells, for regenerative medicine applications
AU - Shtrichman, Ronit
AU - Zeevi-Levin, Naama
AU - Zaid, Rinat
AU - Barak, Efrat
AU - Fishman, Bettina
AU - Ziskind, Anna
AU - Shulman, Rita
AU - Novak, Atara
AU - Avrahami, Ron
AU - Livne, Erella
AU - Lowenstein, Lior
AU - Zussman, Eyal
AU - Itskovitz-Eldor, Joseph
N1 - Publisher Copyright:
© Copyright 2014, Mary Ann Liebert, Inc.
PY - 2014/10
Y1 - 2014/10
N2 - Extracellular matrix (ECM) has been utilized as a biological scaffold for tissue engineering applications in a variety of body systems, due to its bioactivity and biocompatibility. In the current study we developed a modified protocol for the efficient and reproducible derivation of mesenchymal progenitor cells (MPCs) from human embryonic stem cells as well as human induced pluripotent stem cells (hiPSCs) originating from hair follicle keratinocytes (HFKTs). ECM was produced from these MPCs and characterized in comparison to adipose mesenchymal stem cell ECM, demonstrating robust ECM generation by the excised HFKT-iPSC-MPCs. Exploiting the advantages of electrospinning we generated two types of electrospun biodegradable nanofiber layers (NFLs), fabricated from polycaprolactone (PCL) and poly(lactic-co-glycolic acid) (PLGA), which provide mechanical support for cell seeding and ECM generation. Elucidating the optimized decellularization treatment we were able to generate an available "off-the-shelf" implantable product (NFL-ECM). Using rat subcutaneous transplantation model we demonstrate that this stem-cell-derived construct is biocompatible and biodegradable and holds great potential for tissue regeneration applications.
AB - Extracellular matrix (ECM) has been utilized as a biological scaffold for tissue engineering applications in a variety of body systems, due to its bioactivity and biocompatibility. In the current study we developed a modified protocol for the efficient and reproducible derivation of mesenchymal progenitor cells (MPCs) from human embryonic stem cells as well as human induced pluripotent stem cells (hiPSCs) originating from hair follicle keratinocytes (HFKTs). ECM was produced from these MPCs and characterized in comparison to adipose mesenchymal stem cell ECM, demonstrating robust ECM generation by the excised HFKT-iPSC-MPCs. Exploiting the advantages of electrospinning we generated two types of electrospun biodegradable nanofiber layers (NFLs), fabricated from polycaprolactone (PCL) and poly(lactic-co-glycolic acid) (PLGA), which provide mechanical support for cell seeding and ECM generation. Elucidating the optimized decellularization treatment we were able to generate an available "off-the-shelf" implantable product (NFL-ECM). Using rat subcutaneous transplantation model we demonstrate that this stem-cell-derived construct is biocompatible and biodegradable and holds great potential for tissue regeneration applications.
UR - http://www.scopus.com/inward/record.url?scp=84911924719&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2013.0705
DO - 10.1089/ten.tea.2013.0705
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C2 - 25185111
AN - SCOPUS:84911924719
SN - 1937-3341
VL - 20
SP - 2756
EP - 2767
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
IS - 19-20
ER -
