Abstract
The formation of adenosine 3′:5′-cyclic monophosphate from ATP by testicular mitochondria of immature and mature rats was increased to the same extent by addition of either human chorionic gonadotropin or luteinizing hormone. Follicle stimulating hormone was found to be more active in stimulating adenylate cyclase activity in testicular mitochondria of immature rats. The stimulatory effect of gonadotropins were not suppressed by Ca++ complexing agent ethylene-glycol-bis-(β-amino-ethyl ether) N,N′-tetra-acetic acid. The detergent Lubrol PX, solubilized 75-80% of the mitochondrial adenylate cyclase. The solubilized enzyme was activated by sodium fluoride but not by gonadotropins. The present results indicate a direct effect of gonadotropins on the adenylate cyclase attached to mitochondrial membranes.
| Original language | English |
|---|---|
| Pages (from-to) | 673-679 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 55 |
| Issue number | 3 |
| DOIs | |
| State | Published - 10 Dec 1973 |
Bibliographical note
Funding Information:75-80s of the adenylate cyclase activity was found in the 160,O OOgs upernatant. The solubilized enzyme was activated by sodium fluoride but not by gonadotropic hormones which stimulate the mitochondrial adenylate cyclase. The activation of the solubilized adenylate cyclase by sodium fluoride is shown in Fig. 1. These results are in good agreement with those obtained with myocardial adenylate cyclase, where the solubilized enzyme was activated by sodium fluoride but not by hormones (19). The results reported here suggest that gonadotropic hormones activate the adenylate cyclase attached to mitochondrial membranes and that the enzyme might have some similarities with the adenylate cyclase found to be associated with other membrane systems of the cell, ACKNOWLEDGEMENTS This study was supported by Ford Foundation Grant No. 67-470. assistance of Mrs M. Kaufman is greatly appreciated.
Funding
75-80s of the adenylate cyclase activity was found in the 160,O OOgs upernatant. The solubilized enzyme was activated by sodium fluoride but not by gonadotropic hormones which stimulate the mitochondrial adenylate cyclase. The activation of the solubilized adenylate cyclase by sodium fluoride is shown in Fig. 1. These results are in good agreement with those obtained with myocardial adenylate cyclase, where the solubilized enzyme was activated by sodium fluoride but not by hormones (19). The results reported here suggest that gonadotropic hormones activate the adenylate cyclase attached to mitochondrial membranes and that the enzyme might have some similarities with the adenylate cyclase found to be associated with other membrane systems of the cell, ACKNOWLEDGEMENTS This study was supported by Ford Foundation Grant No. 67-470. assistance of Mrs M. Kaufman is greatly appreciated.
| Funders | Funder number |
|---|---|
| Ford Foundation | 67-470 |