Abstract
The interrelationship between the effect of serum on the induction of porphyrin synthesis, intracellular porphyrin accumulation and photodynamic sensitization of human K562 cells is described. Endogenous porphyrins, synthesized from supplemented 5-amino levulinic acid (5-ALA), were shown to accumulate in the cells, while an addition of serum triggered porphyrin translocation from the cell to the serum. In order to enhance porphyrin accumulation in the cells themselves, they were further stimulated by EDTA, which in combination with 5-ALA reduces Fe++ cellular content. The higher porphyrin cellular content under EDTA and 5-ALA induction was exploited to photoinactivate the human leukemic cells by more then 3 orders of magnitude.
| Original language | English |
|---|---|
| Pages (from-to) | 127-131 |
| Number of pages | 5 |
| Journal | Cancer Letters |
| Volume | 65 |
| Issue number | 2 |
| DOIs | |
| State | Published - 14 Aug 1992 |
Bibliographical note
Funding Information:This researchw as supportedb y a grantt o Z.M. from the Health SciencesC enter Yad Hanadiv and the Charles Krown Research Fund, of the Bar-Ilan University.
Funding
This researchw as supportedb y a grantt o Z.M. from the Health SciencesC enter Yad Hanadiv and the Charles Krown Research Fund, of the Bar-Ilan University.
| Funders | Funder number |
|---|---|
| Charles Krown Research Fund | |
| Health SciencesC enter Yad Hanadiv | |
| Bar-Ilan University |
Keywords
- K562 leukemia
- aminolevulinic acid
- endogenous porphyrin
- photosensitization
