The DC-SIGNR 7/5 genotype is associated with high dendritic cell counts and their subsets in patients infected with HIV-1

Omkar Chaudhary; Manju Bala; Jasbir Singh; Anjali Hazarika; Rajesh Kumar; Kalpana Luthra

doi:10.1007/s10875-013-9868-3

The DC-SIGNR 7/5 genotype is associated with high dendritic cell counts and their subsets in patients infected with HIV-1

Omkar Chaudhary
, Manju Bala
, Jasbir Singh
, Anjali Hazarika
, Rajesh Kumar
, Kalpana Luthra

All India Institute of Medical Sciences, New Delhi
Vardhman Mahavir Medical College & Safdarjung Hospital
Kurukshetra University
Society for Promotion of Youth and Masses

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Purpose: The aim of this study was to assess peripheral blood dendritic cell (DC) frequencies and Dendritic Cell-specific intracellular adhesion molecule 3 grabbing non-integrin related (DC-SIGNR) genotyping in healthy individuals, injecting drug users and HIV-1 infected individuals and correlate with different clinical parameters from north India. Methods: Blood from 30 seronegative healthy individuals, 30 injecting drug users, and 30 patients infected with HIV-1 from North India were collected. Peripheral blood DC frequencies were determined by flow cytometry and repeat region polymorphism in DC-SIGNR was performed by PCR. Results: There was a significantly lower number of DCs and their subsets in patients infected with HIV-1 compared to injecting drug users and healthy individuals. A significant positive correlation of DCs and their subsets with CD4⁺ T cells and negative correlation with HIV-1 viral load was found. A salient finding of this study was the association of the heterozygous 7/5 DC-SIGNR genotypes with higher percentage of DCs and their subsets and higher CD4⁺ T cell counts and lower viral load compared to the homozygous 7/7 DC-SIGNR genotypes in patients infected with HIV-1. Conclusions: This is the first study to assess the DC subsets and its association with DC-SIGNR polymorphism in injecting drug users and HIV-1 infected patients and suggests the protective role of 7/5 DC-SIGNR genotypes in HIV-1 infection.

Original language	English
Pages (from-to)	788-797
Number of pages	10
Journal	Journal of Clinical Immunology
Volume	33
Issue number	4
DOIs	https://doi.org/10.1007/s10875-013-9868-3
State	Published - May 2013
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments We thank the study participants. This work was supported by a grant from the Department of Science and Technology (Grant no. - SR/50/HS-0037/2010). We also thank Indian Council of Medical Research, New Delhi, for providing senior research fellowship.

Funding

Acknowledgments We thank the study participants. This work was supported by a grant from the Department of Science and Technology (Grant no. - SR/50/HS-0037/2010). We also thank Indian Council of Medical Research, New Delhi, for providing senior research fellowship.

Funders	Funder number
Department of Science and Technology, Ministry of Science and Technology, India	SR/50/HS-0037/2010
Indian Council of Medical Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Dendritic cells
HIV-1
North India
flow cytometry
injecting drug users

Access to Document

10.1007/s10875-013-9868-3

Cite this

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title = "The DC-SIGNR 7/5 genotype is associated with high dendritic cell counts and their subsets in patients infected with HIV-1",

abstract = "Purpose: The aim of this study was to assess peripheral blood dendritic cell (DC) frequencies and Dendritic Cell-specific intracellular adhesion molecule 3 grabbing non-integrin related (DC-SIGNR) genotyping in healthy individuals, injecting drug users and HIV-1 infected individuals and correlate with different clinical parameters from north India. Methods: Blood from 30 seronegative healthy individuals, 30 injecting drug users, and 30 patients infected with HIV-1 from North India were collected. Peripheral blood DC frequencies were determined by flow cytometry and repeat region polymorphism in DC-SIGNR was performed by PCR. Results: There was a significantly lower number of DCs and their subsets in patients infected with HIV-1 compared to injecting drug users and healthy individuals. A significant positive correlation of DCs and their subsets with CD4+ T cells and negative correlation with HIV-1 viral load was found. A salient finding of this study was the association of the heterozygous 7/5 DC-SIGNR genotypes with higher percentage of DCs and their subsets and higher CD4+ T cell counts and lower viral load compared to the homozygous 7/7 DC-SIGNR genotypes in patients infected with HIV-1. Conclusions: This is the first study to assess the DC subsets and its association with DC-SIGNR polymorphism in injecting drug users and HIV-1 infected patients and suggests the protective role of 7/5 DC-SIGNR genotypes in HIV-1 infection.",

keywords = "Dendritic cells, HIV-1, North India, flow cytometry, injecting drug users",

author = "Omkar Chaudhary and Manju Bala and Jasbir Singh and Anjali Hazarika and Rajesh Kumar and Kalpana Luthra",

note = "Funding Information: Acknowledgments We thank the study participants. This work was supported by a grant from the Department of Science and Technology (Grant no. - SR/50/HS-0037/2010). We also thank Indian Council of Medical Research, New Delhi, for providing senior research fellowship.",

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AU - Bala, Manju

AU - Singh, Jasbir

AU - Hazarika, Anjali

AU - Kumar, Rajesh

AU - Luthra, Kalpana

N1 - Funding Information: Acknowledgments We thank the study participants. This work was supported by a grant from the Department of Science and Technology (Grant no. - SR/50/HS-0037/2010). We also thank Indian Council of Medical Research, New Delhi, for providing senior research fellowship.

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N2 - Purpose: The aim of this study was to assess peripheral blood dendritic cell (DC) frequencies and Dendritic Cell-specific intracellular adhesion molecule 3 grabbing non-integrin related (DC-SIGNR) genotyping in healthy individuals, injecting drug users and HIV-1 infected individuals and correlate with different clinical parameters from north India. Methods: Blood from 30 seronegative healthy individuals, 30 injecting drug users, and 30 patients infected with HIV-1 from North India were collected. Peripheral blood DC frequencies were determined by flow cytometry and repeat region polymorphism in DC-SIGNR was performed by PCR. Results: There was a significantly lower number of DCs and their subsets in patients infected with HIV-1 compared to injecting drug users and healthy individuals. A significant positive correlation of DCs and their subsets with CD4+ T cells and negative correlation with HIV-1 viral load was found. A salient finding of this study was the association of the heterozygous 7/5 DC-SIGNR genotypes with higher percentage of DCs and their subsets and higher CD4+ T cell counts and lower viral load compared to the homozygous 7/7 DC-SIGNR genotypes in patients infected with HIV-1. Conclusions: This is the first study to assess the DC subsets and its association with DC-SIGNR polymorphism in injecting drug users and HIV-1 infected patients and suggests the protective role of 7/5 DC-SIGNR genotypes in HIV-1 infection.

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The DC-SIGNR 7/5 genotype is associated with high dendritic cell counts and their subsets in patients infected with HIV-1

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

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