Abstract
Cloned cDNA probes were used to measure the accumulation of myosin heavy chain, myosin light chain 2, and actin mRNA during differentiation of rat skeletal muscle cell cultures. This was compared with the changes in the rate of synthesis of the corresponding proteins. Accumulation of those mRNA sequences was detectable a few hours before the onset of the phase of cell fusion; however, the main increase in hybridizable RNA occurred during the phase of rapid cell fusion. A close correlation was found between the amounts of mRNAs coding for these proteins and the rate of synthesis of the proteins. The results suggest that the activation of stored mRNA is not a major mechanism for controlling the time at which these proteins are synthesized.
| Original language | English |
|---|---|
| Pages (from-to) | 483-492 |
| Number of pages | 10 |
| Journal | Developmental Biology |
| Volume | 86 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 1981 |
| Externally published | Yes |
Bibliographical note
Funding Information:We wish to thank Ms. Mickaela Miller, Zehava Levi, and Sara Neu-man for their excellent assistance in this work, and Dr. J. Calvo for his valuable discussions and help in the editing of the manuscript. This work was supported by a grant from the Muscular Dystrophy Association (II. S. A.) and by the National Institutes of Health under Grant ROl GM 22767.
Funding
We wish to thank Ms. Mickaela Miller, Zehava Levi, and Sara Neu-man for their excellent assistance in this work, and Dr. J. Calvo for his valuable discussions and help in the editing of the manuscript. This work was supported by a grant from the Muscular Dystrophy Association (II. S. A.) and by the National Institutes of Health under Grant ROl GM 22767.
| Funders | Funder number |
|---|---|
| National Institutes of Health | |
| National Institute of General Medical Sciences | R01GM022767 |
| Muscular Dystrophy Association |