The bactericidal activity of a deuteroporphyrin-hemin mixture on gram-positive bacteria. A microbiological and spectroscopic study

Zvi Malik; Hava Ladan; Yeshayahu Nitzan; Benjamin Ehrenberg

doi:10.1016/1011-1344(90)85115-D

The bactericidal activity of a deuteroporphyrin-hemin mixture on gram-positive bacteria. A microbiological and spectroscopic study

Zvi Malik, Hava Ladan, Yeshayahu Nitzan, Benjamin Ehrenberg

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The combined antibacterial activity of various porphyrins with hemin on Gram-positive bacteria was studied. Protoporphyrin, hematoporphyrin derivative and deuteroporphyrin show only a marginal inhibitory effect in the dark. However, hemin has a strong cytotoxic effect which is independent of illumination and is equally strong in the dark. The disadvantage of hemin treatment is that it is temporary. In this study, we have demonstrated that a combination of deuteroporphyrin and hemin has a unique cytotoxic activity on Staphytococcus aureus, Streptococcus faecalis and Bacillus cereus. The effect of the combined compound is stronger than that of the separate constituents, and is as strong in the dark as in the light. Only 0.005% of the initial S. aureus population survive after a 2 h treatment. Absorption and fluorescence spectra of hemin-deuteroporphyrin mixtures in water and liposomes suggest the formation of a species with spectroscopic properties which are different from those of the two constituents.

Original language	English
Pages (from-to)	419-430
Number of pages	12
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	6
Issue number	4
DOIs	https://doi.org/10.1016/1011-1344(90)85115-D
State	Published - Aug 1990

Bibliographical note

Funding Information:
The authors wish to honour the memory of an excellent technical assistant, Mrs. S. Gozhansky, who helped with the experimental work in this study and died during submission of the manuscript. This work was supported in part by the Health Sciences Research Centre, Department of Life Science, Bar-Ilan University.

Funding

The authors wish to honour the memory of an excellent technical assistant, Mrs. S. Gozhansky, who helped with the experimental work in this study and died during submission of the manuscript. This work was supported in part by the Health Sciences Research Centre, Department of Life Science, Bar-Ilan University.

Funders	Funder number
Department of Life Science, Bar-Ilan University
Health Sciences Research Centre

Keywords

Bacillus cereus
Deuteroporphyrin
Staphylococcus aureus
Streptococcus faecalis
fluorescence.
hematoporphyrin
hemin
protoporphyrin

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10.1016/1011-1344(90)85115-D

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title = "The bactericidal activity of a deuteroporphyrin-hemin mixture on gram-positive bacteria. A microbiological and spectroscopic study",

abstract = "The combined antibacterial activity of various porphyrins with hemin on Gram-positive bacteria was studied. Protoporphyrin, hematoporphyrin derivative and deuteroporphyrin show only a marginal inhibitory effect in the dark. However, hemin has a strong cytotoxic effect which is independent of illumination and is equally strong in the dark. The disadvantage of hemin treatment is that it is temporary. In this study, we have demonstrated that a combination of deuteroporphyrin and hemin has a unique cytotoxic activity on Staphytococcus aureus, Streptococcus faecalis and Bacillus cereus. The effect of the combined compound is stronger than that of the separate constituents, and is as strong in the dark as in the light. Only 0.005% of the initial S. aureus population survive after a 2 h treatment. Absorption and fluorescence spectra of hemin-deuteroporphyrin mixtures in water and liposomes suggest the formation of a species with spectroscopic properties which are different from those of the two constituents.",

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author = "Zvi Malik and Hava Ladan and Yeshayahu Nitzan and Benjamin Ehrenberg",

note = "Funding Information: The authors wish to honour the memory of an excellent technical assistant, Mrs. S. Gozhansky, who helped with the experimental work in this study and died during submission of the manuscript. This work was supported in part by the Health Sciences Research Centre, Department of Life Science, Bar-Ilan University.",

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doi = "10.1016/1011-1344(90)85115-D",

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AU - Ehrenberg, Benjamin

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N2 - The combined antibacterial activity of various porphyrins with hemin on Gram-positive bacteria was studied. Protoporphyrin, hematoporphyrin derivative and deuteroporphyrin show only a marginal inhibitory effect in the dark. However, hemin has a strong cytotoxic effect which is independent of illumination and is equally strong in the dark. The disadvantage of hemin treatment is that it is temporary. In this study, we have demonstrated that a combination of deuteroporphyrin and hemin has a unique cytotoxic activity on Staphytococcus aureus, Streptococcus faecalis and Bacillus cereus. The effect of the combined compound is stronger than that of the separate constituents, and is as strong in the dark as in the light. Only 0.005% of the initial S. aureus population survive after a 2 h treatment. Absorption and fluorescence spectra of hemin-deuteroporphyrin mixtures in water and liposomes suggest the formation of a species with spectroscopic properties which are different from those of the two constituents.

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The bactericidal activity of a deuteroporphyrin-hemin mixture on gram-positive bacteria. A microbiological and spectroscopic study

Abstract

Bibliographical note

Funding

Keywords

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