The antibacterial activity of haemin compared with cobalt, zinc and magnesium protoporphyrin and its effect on pottassium loss and ultrastructure of Staphylococcus aureus

H. Ladan; Y. Nitzan; Z. Malik

doi:10.1111/j.1574-6968.1993.tb06444.x

The antibacterial activity of haemin compared with cobalt, zinc and magnesium protoporphyrin and its effect on pottassium loss and ultrastructure of Staphylococcus aureus

H. Ladan, Y. Nitzan, Z. Malik

Bar-Ilan University

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36 Scopus citations

Abstract

The unique antibacterial properties of Fe-protoporphyrin (haemin) on Staphylococcus aureus, compared to Co-protoprophyrin (Co-PP), Mg-protoporphyrin (Mg-PP) and Zn-protoporphyrin (Zn-PP) are described. Only haemin (20 μM) exhibits a strong light-independent antibacterial effect on S. aureus; the other metalloporphyrins, Co-PP, Mg-PP or Zn-PP, have no antibacterial effect in the dark. Only light photosensitization of Mg-PP-treated cells resulted in the inhibition of the bacterial growth, while Co-PP or Zn-PP were photodynamically inactive. A notable effect of haemin on inactivation of S. aureus was the induction of immediate ion fluxes as determined by X-ray microanalysis (XRMA) of fast-frozen cells. A marked efflux of K (96%) and Cl (94%) was expressed immediately as determined by X-ray microanalysis of S. aureus cells treated with haemin for 5 min. Only 48% loss of Na was detected in the cells under these treatment conditions, while P content was increased by 150%. Electron microscopy analysis revealed the appearance of a mesosome-like structure connected to the new septa, filmentous chromosome and arrays of aggregated ribosomes in the cytoplasm. We propose that haemin has multiple cellular targets for its oxidative effect in S. aureus.

Original language	English
Pages (from-to)	173-177
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	112
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06444.x
State	Published - 1 Sep 1993

Bibliographical note

Funding Information:
We wish to thank Ms. R. Dror and Ms. J. Hanania for their skillful assistance during this research. This research was supported by a grant to Z.M. and Y.N. from the Health Sciences Center, Yad Hanadiv and the Charles Krown Research Fund, of the Bar-Ilan University.

Funding

We wish to thank Ms. R. Dror and Ms. J. Hanania for their skillful assistance during this research. This research was supported by a grant to Z.M. and Y.N. from the Health Sciences Center, Yad Hanadiv and the Charles Krown Research Fund, of the Bar-Ilan University.

Funders	Funder number
Charles Krown Research Fund
Health Sciences Center, Yad Hanadiv
Bar-Ilan University

Keywords

Haemin
Metalloporphyrin
Potassium
Staphylococcus aureus
Ultrastructure
X-ray microanalysis

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10.1111/j.1574-6968.1993.tb06444.x

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title = "The antibacterial activity of haemin compared with cobalt, zinc and magnesium protoporphyrin and its effect on pottassium loss and ultrastructure of Staphylococcus aureus",

abstract = "The unique antibacterial properties of Fe-protoporphyrin (haemin) on Staphylococcus aureus, compared to Co-protoprophyrin (Co-PP), Mg-protoporphyrin (Mg-PP) and Zn-protoporphyrin (Zn-PP) are described. Only haemin (20 μM) exhibits a strong light-independent antibacterial effect on S. aureus; the other metalloporphyrins, Co-PP, Mg-PP or Zn-PP, have no antibacterial effect in the dark. Only light photosensitization of Mg-PP-treated cells resulted in the inhibition of the bacterial growth, while Co-PP or Zn-PP were photodynamically inactive. A notable effect of haemin on inactivation of S. aureus was the induction of immediate ion fluxes as determined by X-ray microanalysis (XRMA) of fast-frozen cells. A marked efflux of K (96\%) and Cl (94\%) was expressed immediately as determined by X-ray microanalysis of S. aureus cells treated with haemin for 5 min. Only 48\% loss of Na was detected in the cells under these treatment conditions, while P content was increased by 150\%. Electron microscopy analysis revealed the appearance of a mesosome-like structure connected to the new septa, filmentous chromosome and arrays of aggregated ribosomes in the cytoplasm. We propose that haemin has multiple cellular targets for its oxidative effect in S. aureus.",

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author = "H. Ladan and Y. Nitzan and Z. Malik",

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N1 - Funding Information: We wish to thank Ms. R. Dror and Ms. J. Hanania for their skillful assistance during this research. This research was supported by a grant to Z.M. and Y.N. from the Health Sciences Center, Yad Hanadiv and the Charles Krown Research Fund, of the Bar-Ilan University.

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The antibacterial activity of haemin compared with cobalt, zinc and magnesium protoporphyrin and its effect on pottassium loss and ultrastructure of Staphylococcus aureus

Abstract

Bibliographical note

Funding

Keywords

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