Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF

C. Kaplinsky; L. Trakhtenbrot; I. Hardan; M. Reichart; M. Daniely; A. Toren; N. Amariglio; G. Rechavi; S. Izraeli

doi:10.1038/sj.bmt.1703902

Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF

C. Kaplinsky, L. Trakhtenbrot, I. Hardan, M. Reichart, M. Daniely, A. Toren, N. Amariglio, G. Rechavi, S. Izraeli

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is frequently used to mobilize CD34+ cells in healthy donors and patient with malignant diseases prior to peripheral blood stem cell (PBSC) harvest. To analyze the effects of rhG-CSF on morphology and genotype of white blood cells, a novel multiparametric cell scanning system that combines morphologic, immune and genotypic analyses of the same cells was used. We report here that tetraploid myeloid cells are present in the peripheral blood of donors treated with rhG-CSF. The tetraploidy was detected in up to 0.6% of differentiated myeloid cells and all observed CD34+ cells were diploid. Thus, short treatment with rhG-CSF of PBSC donors induces numerical chromosomal alterations in a small subset of mature myeloid cells.

Original language	English
Pages (from-to)	31-34
Number of pages	4
Journal	Bone Marrow Transplantation
Volume	32
Issue number	1
DOIs	https://doi.org/10.1038/sj.bmt.1703902
State	Published - Jul 2003
Externally published	Yes

Keywords

Cytogenetics
G-CSF
Peripheral stem cell harvest
Peripheral stem cell transplantation
Tetraploidy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/sj.bmt.1703902

Cite this

@article{a36a69b5129147fd99f88c7c14284f6e,

title = "Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF",

abstract = "Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is frequently used to mobilize CD34+ cells in healthy donors and patient with malignant diseases prior to peripheral blood stem cell (PBSC) harvest. To analyze the effects of rhG-CSF on morphology and genotype of white blood cells, a novel multiparametric cell scanning system that combines morphologic, immune and genotypic analyses of the same cells was used. We report here that tetraploid myeloid cells are present in the peripheral blood of donors treated with rhG-CSF. The tetraploidy was detected in up to 0.6% of differentiated myeloid cells and all observed CD34+ cells were diploid. Thus, short treatment with rhG-CSF of PBSC donors induces numerical chromosomal alterations in a small subset of mature myeloid cells.",

keywords = "Cytogenetics, G-CSF, Peripheral stem cell harvest, Peripheral stem cell transplantation, Tetraploidy",

author = "C. Kaplinsky and L. Trakhtenbrot and I. Hardan and M. Reichart and M. Daniely and A. Toren and N. Amariglio and G. Rechavi and S. Izraeli",

year = "2003",

month = jul,

doi = "10.1038/sj.bmt.1703902",

language = "אנגלית",

volume = "32",

pages = "31--34",

journal = "Bone Marrow Transplantation",

issn = "0268-3369",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF

AU - Kaplinsky, C.

AU - Trakhtenbrot, L.

AU - Hardan, I.

AU - Reichart, M.

AU - Daniely, M.

AU - Toren, A.

AU - Amariglio, N.

AU - Rechavi, G.

AU - Izraeli, S.

PY - 2003/7

Y1 - 2003/7

N2 - Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is frequently used to mobilize CD34+ cells in healthy donors and patient with malignant diseases prior to peripheral blood stem cell (PBSC) harvest. To analyze the effects of rhG-CSF on morphology and genotype of white blood cells, a novel multiparametric cell scanning system that combines morphologic, immune and genotypic analyses of the same cells was used. We report here that tetraploid myeloid cells are present in the peripheral blood of donors treated with rhG-CSF. The tetraploidy was detected in up to 0.6% of differentiated myeloid cells and all observed CD34+ cells were diploid. Thus, short treatment with rhG-CSF of PBSC donors induces numerical chromosomal alterations in a small subset of mature myeloid cells.

AB - Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is frequently used to mobilize CD34+ cells in healthy donors and patient with malignant diseases prior to peripheral blood stem cell (PBSC) harvest. To analyze the effects of rhG-CSF on morphology and genotype of white blood cells, a novel multiparametric cell scanning system that combines morphologic, immune and genotypic analyses of the same cells was used. We report here that tetraploid myeloid cells are present in the peripheral blood of donors treated with rhG-CSF. The tetraploidy was detected in up to 0.6% of differentiated myeloid cells and all observed CD34+ cells were diploid. Thus, short treatment with rhG-CSF of PBSC donors induces numerical chromosomal alterations in a small subset of mature myeloid cells.

KW - Cytogenetics

KW - G-CSF

KW - Peripheral stem cell harvest

KW - Peripheral stem cell transplantation

KW - Tetraploidy

UR - http://www.scopus.com/inward/record.url?scp=0037817611&partnerID=8YFLogxK

U2 - 10.1038/sj.bmt.1703902

DO - 10.1038/sj.bmt.1703902

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 12815475

AN - SCOPUS:0037817611

SN - 0268-3369

VL - 32

SP - 31

EP - 34

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

IS - 1

ER -

Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this