TY - JOUR
T1 - Testing human sperm chemotaxis
T2 - How to detect biased motion in population assays
AU - Armon, Leah
AU - Caplan, S. Roy
AU - Eisenbach, Michael
AU - Friedrich, Benjamin M.
PY - 2012/3/8
Y1 - 2012/3/8
N2 - Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming.
AB - Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming.
UR - http://www.scopus.com/inward/record.url?scp=84857842661&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0032909
DO - 10.1371/journal.pone.0032909
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C2 - 22412947
AN - SCOPUS:84857842661
SN - 1932-6203
VL - 7
JO - PLoS ONE
JF - PLoS ONE
IS - 3
M1 - e32909
ER -