Testicular sperm retrieval: What should we expect from the fresh and subsequent cryopreserved sperm injection?

Adva Aizer, Olga Dratviman-Storobinsky, Meirav Noach-Hirsh, Sarah Konopnicki, Alon Lazarovich, Gil Raviv, Raoul Orvieto

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We sought to compare ICSI outcomes of cycle using fresh versus thawed TESE spermatozoa obtained during the previous fresh TESE. All consecutive couples undergoing ICSI cycles using fresh TESE spermatozoa, followed by ICSI cycle using cryopreserved sperm remaining from the previous fresh TESE procedure were included. Ovarian stimulation (OS)/laboratory variables and cycle outcome were assessed and compared between those utilising fresh versus thawed TESE spermatozoa. Seventy-five couples were evaluated, with no in-between groups differences in OS nor embryological variables. While implantation and LBR per embryo transfer were nonsignificantly higher in the frozen as compared to the fresh TESE, there was a trend towards higher LBRs per patient in the frozen TESE group. The cumulative miscarriage rate (4% versus 14.7%, p <.022 respectively) was significantly lower and the cumulative LBR (34.7% versus 16%, p <.007 respectively) was significantly higher using frozen TESE spermatozoa. Moreover, significantly higher proportion of frozen TESE sperm samples used pentoxifylline to enhance sperm motility. In conclusion, the results of ICSI cycles using frozen TESE spermatozoa are as good, or even better than using fresh TESE spermatozoa. Further studies are required to explore the factors responsible for the improved ICSI outcome, while using frozen versus fresh TESE sperm samples.

Original languageEnglish
Article numbere13849
Issue number1
StatePublished - Feb 2021
Externally publishedYes

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  • ICSI
  • azoospermia
  • fresh
  • frozen-thawed
  • live birth rate
  • testicular sperm retrieval


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