TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription

Jer Yuan Hsu, Tamar Juven-Gershon, Michael T. Marr, Kevin J. Wright, Robert Tjian, James T. Kadonaga

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this circuit, TBP activates TATA-dependent transcription and represses DPE-dependent transcription, whereas Mot1 and NC2 block TBP function and thus repress TATA-dependent transcription and activate DPE-dependent transcription. This regulatory circuit is likely to be one means by which biological networks can transmit transcriptional signals, such as those from DPE-specific and TATA-specific enhancers, via distinct pathways.

Original languageEnglish
Pages (from-to)2353-2358
Number of pages6
JournalGenes and Development
Volume22
Issue number17
DOIs
StatePublished - 1 Sep 2008
Externally publishedYes

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM041249

    Keywords

    • Core promoter
    • Mot1
    • NC2
    • RNA polymerase II
    • TBP
    • Transcription

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