Targeted disruption of mouse EGF receptor: Effect of genetic background on mutant phenotype

David W. Threadgill, Andrzej A. Dlugosz, Laura A. Hansen, Tamar Tennenbaum, Ulrike Lichti, Della Yee, Christian LaMantia, Tracy Mourton, Karl Herrup, Raymond C. Harris, John A. Barnard, Stuart H. Yuspa, Robert J. Coffey, Terry Magnuson

Research output: Contribution to journalArticlepeer-review

1290 Scopus citations


Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.

Original languageEnglish
Pages (from-to)230-234
Number of pages5
Issue number5221
StatePublished - 14 Jul 1995
Externally publishedYes


FundersFunder number
National Institute of General Medical SciencesF32GM014630
Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentR01HD026722


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