TY - JOUR
T1 - T-Cell inactivation and immunosuppressive activity induced by HIV gp41 via novel interacting motif
AU - Bloch, Itai
AU - Quintana, Francisco J.
AU - Gerber, Doron
AU - Cohen, Tomer
AU - Cohen, Irun R.
AU - Shai, Yechiel
PY - 2007/2
Y1 - 2007/2
N2 - Fusion peptide (FP) of the HIV gp41 molecule inserts into the T cell membrane during virus-cell fusion. FP also blocks the TCR/CD3 interaction needed for antigen-triggered T cell activation. Here we used in vitro (fluorescence and immunoprecipitation), in vivo (T cell mediated autoimmune disease adjuvant arthritis), and in silico methods to identify the FP-TCR novel interaction motif: the α-helical transmembrane domain (TMD) of the TCR α chain, and the β-sheet 5-13 region of the 16 N-terminal aa of FP (FP 1-16). Deciphering the molecular mechanism of the immunosuppressive activity of FP provides a new potential target to overcome the immunosuppressant activity of HIV, and in addition a tool for down-regulating immune mediated inflammation.
AB - Fusion peptide (FP) of the HIV gp41 molecule inserts into the T cell membrane during virus-cell fusion. FP also blocks the TCR/CD3 interaction needed for antigen-triggered T cell activation. Here we used in vitro (fluorescence and immunoprecipitation), in vivo (T cell mediated autoimmune disease adjuvant arthritis), and in silico methods to identify the FP-TCR novel interaction motif: the α-helical transmembrane domain (TMD) of the TCR α chain, and the β-sheet 5-13 region of the 16 N-terminal aa of FP (FP 1-16). Deciphering the molecular mechanism of the immunosuppressive activity of FP provides a new potential target to overcome the immunosuppressant activity of HIV, and in addition a tool for down-regulating immune mediated inflammation.
KW - Membrane proteins
KW - Peptide-membrane interaction
KW - Protein-membrane interaction
KW - Recognition within membranes
KW - Transmembrane domain
UR - http://www.scopus.com/inward/record.url?scp=33846807210&partnerID=8YFLogxK
U2 - 10.1096/fj.06-7061com
DO - 10.1096/fj.06-7061com
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C2 - 17185749
AN - SCOPUS:33846807210
SN - 0892-6638
VL - 21
SP - 393
EP - 401
JO - FASEB Journal
JF - FASEB Journal
IS - 2
ER -
