Synthesis of lipase polymer hybrids with retained or enhanced activity using the grafting-from strategy

Marina Kovaliov; Michael L. Allegrezza; Bertram Richter; Dominik Konkolewicz; Saadyah Averick

doi:10.1016/j.polymer.2018.01.026

Synthesis of lipase polymer hybrids with retained or enhanced activity using the grafting-from strategy

Marina Kovaliov
, Michael L. Allegrezza
, Bertram Richter
, Dominik Konkolewicz
, Saadyah Averick

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

We report the synthesis of new polymer-protein conjugates using a grafting-from method employing photoinduced electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. Lipase, an industrially important enzyme, was modified with a reversible addition-fragmentation (RAFT) chain transfer agent (CTA) using activated ester chemistry. To study the effects of polymer composition and molecular weight on the enzyme activity, a hydrophilic and hydrophobic polymer were grafted at three different polymer lengths. We found a significant impact of polymer composition on enzyme activity with hydrophobic polymer substantially increasing enzyme activity.

Original language	English
Pages (from-to)	338-345
Number of pages	8
Journal	Polymer
Volume	137
DOIs	https://doi.org/10.1016/j.polymer.2018.01.026
State	Published - 14 Feb 2018
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2018 Elsevier Ltd

Funding

We gratefully acknowledge The Army Research Office (Award# 68271-CH) Young Investigator Program ( W911NF-17-1-0015 ) and the Neuroscience Institute at AHN for funding NMR measurements and instrumentation at CMU which was partially supported by NSF ( CHE-0130903 and CHE-1039870 ). The MALDI-TOF data were collected using instrumentation purchased with NSF grant award CHE-0839233 . We gratefully acknowledge The Army Research Office (Award# 68271-CH) Young Investigator Program (W911NF-17-1-0015) and the Neuroscience Institute at AHN for funding NMR measurements and instrumentation at CMU which was partially supported by NSF (CHE-0130903 and CHE-1039870). The MALDI-TOF data were collected using instrumentation purchased with NSF grant award CHE-0839233.

Funders	Funder number
National Science Foundation	CHE-1039870, CHE-0130903
Army Research Office	W911NF-17-1-0015, 68271-CH
Carnegie Mellon University
Neuroscience Institute, University of Tennessee
Allegheny Health Network
National Stroke Foundation	CHE-0839233

Keywords

Grafting-from
Lipase
PET-RAFT
Protein-polymer hybrid
RDRP

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10.1016/j.polymer.2018.01.026

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abstract = "We report the synthesis of new polymer-protein conjugates using a grafting-from method employing photoinduced electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. Lipase, an industrially important enzyme, was modified with a reversible addition-fragmentation (RAFT) chain transfer agent (CTA) using activated ester chemistry. To study the effects of polymer composition and molecular weight on the enzyme activity, a hydrophilic and hydrophobic polymer were grafted at three different polymer lengths. We found a significant impact of polymer composition on enzyme activity with hydrophobic polymer substantially increasing enzyme activity.",

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author = "Marina Kovaliov and Allegrezza, \{Michael L.\} and Bertram Richter and Dominik Konkolewicz and Saadyah Averick",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

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AU - Richter, Bertram

AU - Konkolewicz, Dominik

AU - Averick, Saadyah

PY - 2018/2/14

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AB - We report the synthesis of new polymer-protein conjugates using a grafting-from method employing photoinduced electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. Lipase, an industrially important enzyme, was modified with a reversible addition-fragmentation (RAFT) chain transfer agent (CTA) using activated ester chemistry. To study the effects of polymer composition and molecular weight on the enzyme activity, a hydrophilic and hydrophobic polymer were grafted at three different polymer lengths. We found a significant impact of polymer composition on enzyme activity with hydrophobic polymer substantially increasing enzyme activity.

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KW - Protein-polymer hybrid

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JO - Polymer

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Synthesis of lipase polymer hybrids with retained or enhanced activity using the grafting-from strategy

Abstract

Bibliographical note

Funding

Keywords

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