Synthesis of Human Interferon β1 in Escherichia coliInfected by a Lambda Phage Recombinant containing a Human Genomic Fragment

Yves MORY; Yuti CHERNAJOVSKY; Sheldon I. FEINSTEIN; Lousia CHEN; Uri NIR; Jean WEISSENBACH; Yves MALPIECE; Pierre TIOLLAIS; David MARKS; Martha LADNER; Clarence COLBY; Michel REVEL

doi:10.1111/j.1432-1033.1981.tb05689.x

Synthesis of Human Interferon β₁ in Escherichia coliInfected by a Lambda Phage Recombinant containing a Human Genomic Fragment

Yves MORY, Yuti CHERNAJOVSKY, Sheldon I. FEINSTEIN, Lousia CHEN, Uri NIR, Jean WEISSENBACH, Yves MALPIECE, Pierre TIOLLAIS, David MARKS, Martha LADNER, Clarence COLBY, Michel REVEL

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Abstract

DNA from a human adult was fragmented by partial digestion with restriction endonuclease EcoRI and cloned in λ Charon 4A. Clone C15, with a human DNA insert of 17 × 10³ bases, was identified as containg a gene for the fibroblast, interferon, interferon β₁. Restriction mapping shows that this gene, located on a 1840‐base EcoRI fragment, is not interrupted by introns. Moreover, we show that this human genomic DNA fragment is able to direct the synthesis of active human interferon β₁ in Escherichia coli. Interferon activity of up to 7 × 10⁶ U/1 was recovered from phage lysates by chromatography on CiBacron blue–Sepharose, and had the same immunological properties and species specificity as interferon produced by human fibroblasts.

Original language	English
Pages (from-to)	197-202
Number of pages	6
Journal	European Journal of Biochemistry
Volume	120
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-1033.1981.tb05689.x
State	Published - Nov 1981
Externally published	Yes

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MORY, Y., CHERNAJOVSKY, Y., FEINSTEIN, S. I., CHEN, L., NIR, U., WEISSENBACH, J., MALPIECE, Y., TIOLLAIS, P., MARKS, D., LADNER, M., COLBY, C., & REVEL, M. (1981). Synthesis of Human Interferon β₁ in Escherichia coliInfected by a Lambda Phage Recombinant containing a Human Genomic Fragment. European Journal of Biochemistry, 120(1), 197-202. https://doi.org/10.1111/j.1432-1033.1981.tb05689.x

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title = "Synthesis of Human Interferon β1 in Escherichia coliInfected by a Lambda Phage Recombinant containing a Human Genomic Fragment",

abstract = "DNA from a human adult was fragmented by partial digestion with restriction endonuclease EcoRI and cloned in λ Charon 4A. Clone C15, with a human DNA insert of 17 × 103 bases, was identified as containg a gene for the fibroblast, interferon, interferon β1. Restriction mapping shows that this gene, located on a 1840‐base EcoRI fragment, is not interrupted by introns. Moreover, we show that this human genomic DNA fragment is able to direct the synthesis of active human interferon β1 in Escherichia coli. Interferon activity of up to 7 × 106 U/1 was recovered from phage lysates by chromatography on CiBacron blue–Sepharose, and had the same immunological properties and species specificity as interferon produced by human fibroblasts.",

author = "Yves MORY and Yuti CHERNAJOVSKY and FEINSTEIN, {Sheldon I.} and Lousia CHEN and Uri NIR and Jean WEISSENBACH and Yves MALPIECE and Pierre TIOLLAIS and David MARKS and Martha LADNER and Clarence COLBY and Michel REVEL",

year = "1981",

month = nov,

doi = "10.1111/j.1432-1033.1981.tb05689.x",

language = "אנגלית",

volume = "120",

pages = "197--202",

journal = "European Journal of Biochemistry",

issn = "0014-2956",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

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MORY, Y, CHERNAJOVSKY, Y, FEINSTEIN, SI, CHEN, L, NIR, U, WEISSENBACH, J, MALPIECE, Y, TIOLLAIS, P, MARKS, D, LADNER, M, COLBY, C & REVEL, M 1981, 'Synthesis of Human Interferon β₁ in Escherichia coliInfected by a Lambda Phage Recombinant containing a Human Genomic Fragment', European Journal of Biochemistry, vol. 120, no. 1, pp. 197-202. https://doi.org/10.1111/j.1432-1033.1981.tb05689.x

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T1 - Synthesis of Human Interferon β1 in Escherichia coliInfected by a Lambda Phage Recombinant containing a Human Genomic Fragment

AU - MORY, Yves

AU - CHERNAJOVSKY, Yuti

AU - FEINSTEIN, Sheldon I.

AU - CHEN, Lousia

AU - NIR, Uri

AU - WEISSENBACH, Jean

AU - MALPIECE, Yves

AU - TIOLLAIS, Pierre

AU - MARKS, David

AU - LADNER, Martha

AU - COLBY, Clarence

AU - REVEL, Michel

PY - 1981/11

Y1 - 1981/11

N2 - DNA from a human adult was fragmented by partial digestion with restriction endonuclease EcoRI and cloned in λ Charon 4A. Clone C15, with a human DNA insert of 17 × 103 bases, was identified as containg a gene for the fibroblast, interferon, interferon β1. Restriction mapping shows that this gene, located on a 1840‐base EcoRI fragment, is not interrupted by introns. Moreover, we show that this human genomic DNA fragment is able to direct the synthesis of active human interferon β1 in Escherichia coli. Interferon activity of up to 7 × 106 U/1 was recovered from phage lysates by chromatography on CiBacron blue–Sepharose, and had the same immunological properties and species specificity as interferon produced by human fibroblasts.

AB - DNA from a human adult was fragmented by partial digestion with restriction endonuclease EcoRI and cloned in λ Charon 4A. Clone C15, with a human DNA insert of 17 × 103 bases, was identified as containg a gene for the fibroblast, interferon, interferon β1. Restriction mapping shows that this gene, located on a 1840‐base EcoRI fragment, is not interrupted by introns. Moreover, we show that this human genomic DNA fragment is able to direct the synthesis of active human interferon β1 in Escherichia coli. Interferon activity of up to 7 × 106 U/1 was recovered from phage lysates by chromatography on CiBacron blue–Sepharose, and had the same immunological properties and species specificity as interferon produced by human fibroblasts.

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Synthesis of Human Interferon β₁ in Escherichia coliInfected by a Lambda Phage Recombinant containing a Human Genomic Fragment

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