Synthesis and characterization of thrombin conjugated γ-Fe 2O3 magnetic nanoparticles for hemostasis

Ofra Ziv, Tammy Lublin-Tennenbaum, Shlomo Margel

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Thrombin is the final protease produced in the clotting pathways. Thrombin has been used in the clinic more than six decades for topical hemostasis and wound management. In human plasma the half-life of thrombin is shorter than 15 seconds due to close control by inhibitors. In order to stabilize thrombin, this enzyme was conjugated covalently and physically to γ-Fe2O 3 magnetic nanoparticles. The physical conjugation was accomplished through adsorption of thrombin to BSA coating on the nanoparticles. The coagulant activity of the covalently bound thrombin was significantly lower than that of the physically adsorbed thrombin. Leakage of the physically bound thrombin into PBS containing 4% HSA was negligible. The physical conjugation of thrombin onto the nanoparticles stabilized the thrombin against its major inhibitor antithrombin III and improved its storage stability. At optimal CaCl2 concentration, the clotting time by the bound thrombin is shorter than that of the free enzyme. This novel conjugated thrombin may be an efficient candidate for topical hemostasis and wound healing.

Original languageEnglish
Pages (from-to)B251-B260
JournalAdvanced Engineering Materials
Volume11
Issue number12
DOIs
StatePublished - Dec 2009

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