Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique

L. Pichat; A. M. Moustier; J. P. Lellouche; J. P. Beaucourt

doi:10.1002/jlcr.2580230706

Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique

L. Pichat
, A. M. Moustier
, J. P. Lellouche
, J. P. Beaucourt

Serv. des Molecules Marquees

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ^1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH₄ led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐³H] waw obtained purified by liquid chromatography and characterized by TLC, ³H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.

Original language	French
Pages (from-to)	723-740
Number of pages	18
Journal	Journal of Labelled Compounds and Radiopharmaceuticals
Volume	23
Issue number	7
DOIs	https://doi.org/10.1002/jlcr.2580230706
State	Published - Jul 1986
Externally published	Yes

Keywords

3‐Hydroxy‐5‐androstene‐17‐one‐[1,2,15‐H]
Catalytic reduction
H‐NMR
dehydroandrostérone‐H
tritium

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10.1002/jlcr.2580230706

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title = "Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique",

abstract = "Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH4 led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐3H] waw obtained purified by liquid chromatography and characterized by TLC, 3H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.",

keywords = "3‐Hydroxy‐5‐androstene‐17‐one‐[1,2,15‐H], Catalytic reduction, H‐NMR, dehydroandrost{\'e}rone‐H, tritium",

author = "L. Pichat and Moustier, \{A. M.\} and Lellouche, \{J. P.\} and Beaucourt, \{J. P.\}",

year = "1986",

month = jul,

doi = "10.1002/jlcr.2580230706",

language = "צרפתית",

volume = "23",

pages = "723--740",

journal = "Journal of Labelled Compounds and Radiopharmaceuticals",

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publisher = "John Wiley and Sons Ltd",

number = "7",

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T1 - Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique

AU - Pichat, L.

AU - Moustier, A. M.

AU - Lellouche, J. P.

AU - Beaucourt, J. P.

PY - 1986/7

Y1 - 1986/7

N2 - Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH4 led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐3H] waw obtained purified by liquid chromatography and characterized by TLC, 3H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.

AB - Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH4 led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐3H] waw obtained purified by liquid chromatography and characterized by TLC, 3H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.

KW - 3‐Hydroxy‐5‐androstene‐17‐one‐[1,2,15‐H]

KW - Catalytic reduction

KW - H‐NMR

KW - dehydroandrostérone‐H

KW - tritium

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JO - Journal of Labelled Compounds and Radiopharmaceuticals

JF - Journal of Labelled Compounds and Radiopharmaceuticals

IS - 7

ER -

Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique

Abstract

Keywords

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