TY - JOUR
T1 - Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique
AU - Pichat, L.
AU - Moustier, A. M.
AU - Lellouche, J. P.
AU - Beaucourt, J. P.
PY - 1986/7
Y1 - 1986/7
N2 - Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH4 led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐3H] waw obtained purified by liquid chromatography and characterized by TLC, 3H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.
AB - Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH4 led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐3H] waw obtained purified by liquid chromatography and characterized by TLC, 3H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.
KW - 3‐Hydroxy‐5‐androstene‐17‐one‐[1,2,15‐H]
KW - Catalytic reduction
KW - H‐NMR
KW - dehydroandrostérone‐H
KW - tritium
UR - http://www.scopus.com/inward/record.url?scp=0022514566&partnerID=8YFLogxK
U2 - 10.1002/jlcr.2580230706
DO - 10.1002/jlcr.2580230706
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AN - SCOPUS:0022514566
SN - 0362-4803
VL - 23
SP - 723
EP - 740
JO - Journal of Labelled Compounds and Radiopharmaceuticals
JF - Journal of Labelled Compounds and Radiopharmaceuticals
IS - 7
ER -