Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique

L. Pichat, A. M. Moustier, J. P. Lellouche, J. P. Beaucourt

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Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH4 led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐3H] waw obtained purified by liquid chromatography and characterized by TLC, 3H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.

Original languageFrench
Pages (from-to)723-740
Number of pages18
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Issue number7
StatePublished - Jul 1986
Externally publishedYes


  • 3‐Hydroxy‐5‐androstene‐17‐one‐[1,2,15‐H]
  • Catalytic reduction
  • H‐NMR
  • dehydroandrostérone‐H
  • tritium

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