Abstract
VLA-1 integrin expressing T cells are more frequent in inflammatory synovial fluids (SF) compared to peripheral blood. Recent studies suggest that VLA-1 expression mainly marks IFNγ+ T cells while excluding both IL-4+ and regulatory FoxP3+ T cells. To further characterize the TCR repertoire of the potentially pathogenic VLA-1+ IFNγ+ T cells, isolated from SF of adult patients with rheumatoid and psoriatic arthritis, we determined the complementarity determining region (CDR)3 spectratypes. Here we show in a cohort of 9 patients that VLA-1+ T cells display a perturbed repertoire that, moreover, differs from that of VLA-1- synovial T cells and even VLA-1+ PB T cells. Importantly, random sequencing of the CDR3 region of the TCR variable β (BV) 6.1 gene of both VLA-1+ and VLA-1- synovial T cells, in one patient, revealed that their sequences were by and large different (29 out of 33 clones). Thus, our results imply that VLA-1+ T cells that infiltrate into inflamed joints represent a partly distinct and highly oligoclonal population of Th1 cells, probably selected by unique antigens.
Original language | English |
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Pages (from-to) | 75-84 |
Number of pages | 10 |
Journal | Clinical Immunology |
Volume | 128 |
Issue number | 1 |
DOIs | |
State | Published - Jul 2008 |
Externally published | Yes |
Bibliographical note
Funding Information:This work was supported in part by an investigator initiated research grant from Biogen Idec to I.B. G.R. holds the Djerasi Chair for Oncology (Sackler School of Medicine, Tel Aviv University). Part of this work was performed in partial fulfillment of the requirements of the MSc of S. Matzri at the Sackler School of Medicine (Tel Aviv University).
Funding
This work was supported in part by an investigator initiated research grant from Biogen Idec to I.B. G.R. holds the Djerasi Chair for Oncology (Sackler School of Medicine, Tel Aviv University). Part of this work was performed in partial fulfillment of the requirements of the MSc of S. Matzri at the Sackler School of Medicine (Tel Aviv University).
Funders | Funder number |
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Porter School of Environmental Studies, Tel Aviv University |
Keywords
- CDR3 length diversity
- Effector and memory T cells
- Inflammatory arthritis
- Integrin
- T cell receptor (TCR)
- TCR repertoire
- Very late activation antigen (VLA-1)