Surface radiolabeling of PEGylated liposomes for biomedical applications

Liposomes, self-Assembled lipid-based nanoparticles, have gained significant attention due to their versatility and potential applications in various biomedical fields. They serve as promising platforms for targeted drug delivery, imaging, and therapeutics. Among the various types of liposomes, radiolabeled liposomes have attracted considerable interest due to their unique capabilities in both therapy and imaging. In therapy, radiolabeled liposomes can effectively transport therapeutic radioactive agents directly to disease sites, allowing for precise and localized treatment. In imaging, radiolabeling enables non-invasive visualization and tracking of liposomes, providing valuable diagnostic information. In this study, we present a technique for surface radiolabeling of liposomes, achieved by introducing a chelating agent onto the liposome surface and optimizing radiolabeling conditions for desired radionuclides. Importantly, our technique allows for the radiolabeling process to be conducted after the liposomes have been formulated according to the desired composition, enabling seamless integration into biomedical research and clinical practice. Our research investigates optimal radiolabeling conditions for different isotopes, ensuring stability and high efficiency. Purification and characterization of the resulting radiolabeled liposomes validate their quality and stability. The findings of this study offer valuable insights for the future advancement and application of radiolabeled liposomes in biomedical research and clinical practice, holding promise for improved therapies and diagnostics.