Suppression of Bacterial Soft-Rot Diseases Utilizing Plant Phenolic Compounds

Iris Yedidia, Janak Raj Joshi, Netaly Khazanov, Hanoch Senderowitz

Research output: Contribution to conferencePaperpeer-review

Abstract

Pectobacteria rely on N-acylhomoserine lactone (AHL) signaling to coordinate cell to cell communication and synchronize virulence in a collective behavior termed quorum sensing (QS). Plants in response produce an array of small molecules, several of which have been suggested as QS inhibitors, yet with no resolved mode of action at the atomic scale. Here, QS mutant analysis identified AHL synthase (ExpI), as a preferred target for QS inhibitors. Salicylic acid (SA), the plant defense signal, and the essential oil carvacrol (CAR), were demonstrated to inhibit the accumulation of AHL molecules at concentrations that did not affect bacterial growth or membrane integrity. The phenolics also impaired biofilm formation, exoenzyme activities and reduced bacterial virulence during infection of potato and calla lily.
Docking simulations suggest that the phytochemicals interfere with QS by binding of SA/CAR to ExpI. The hypothesis was confirmed by Isothermal Titration Calorimetry and Microscale thermophoresis that demonstrated direct binding between the phenolics and purified ExpI. Furthermore, computational Ala scan served to design an ExpI mutant with a predicted weaker affinity to both ligands. Indeed, the computational predictions were confirmed with lower affinity values of SA and CAR to the mutant protein. The direct binding between phytochemicals and bacterial AHL synthase, reinforce interkingdom signaling between plant molecules and bacterial communication systems.
Original languageAmerican English
StatePublished - 2018
EventPlant Health in a Global Economy, ICPP Boston: 11th International Congress of Plant Pathology - Boston, United States
Duration: 29 Jul 20183 Aug 2018
Conference number: 11
https://www.apsnet.org/meetings/annual/meetingarchives/ICPP2018/Pages/default.aspx

Other

OtherPlant Health in a Global Economy, ICPP Boston
Country/TerritoryUnited States
CityBoston
Period29/07/183/08/18
Internet address

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