Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging

Vicente Micó, Zeev Zalevsky

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The proposed approach can be applied when imaging essentially transparent (low-concentration dilutions ) and static (slow dynamics) samples. Validation of the method for both a synthetic object (U.S. Air Force resolution test) to quantify the resolution improvement and a biological specimen (sperm cells biosample) are reported showing the generation of high synthetic numerical aperture values working without lenses.

Original languageEnglish
Article number046027
JournalJournal of Biomedical Optics
Issue number4
StatePublished - Jul 2010

Bibliographical note

Funding Information:
Part of this work has been supported through grants from the Spanish Ministerio de Educación y Ciencia under the Project No. FIS2007-60626.


  • Digital imaging
  • Digital recording
  • Holography
  • Microscopy
  • Resolution
  • Synthetic apertures


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