Abstract
Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.
Original language | English |
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Title of host publication | Nano-Biotechnology for Biomedical and Diagnostic Research |
Editors | Eran Zahavy, Shmuel Yitzhaki, Arie Ordentlich, Avigdor Shafferman |
Pages | 17-21 |
Number of pages | 5 |
DOIs | |
State | Published - 2011 |
Externally published | Yes |
Publication series
Name | Advances in Experimental Medicine and Biology |
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Volume | 733 |
ISSN (Print) | 0065-2598 |
Bibliographical note
Funding Information:This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). Jörg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).
Funding
This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). Jörg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).
Funders | Funder number |
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National Institutes of Health | 1R01GM086197 |
National Institute of Biomedical Imaging and Bioengineering | R01EB000312 |
Human Frontier Science Program | RGP46/2006 |
Deutsche Forschungsgemeinschaft | DE 1591/1 1 |
Bundesministerium für Bildung und Forschung | FKZ 13N9236 |
Keywords
- Correlation function
- Statistical analysis
- Superresolution