Superresolution optical fluctuation imaging (SOFI)

Thomas Dertinger; Ryan Colyer; Robert Vogel; Mike Heilemann; Markus Sauer; Jörg Enderlein; Shimon Weiss

doi:10.1007/978-94-007-2555-3-2

Superresolution optical fluctuation imaging (SOFI)

Thomas Dertinger, Ryan Colyer, Robert Vogel, Mike Heilemann, Markus Sauer, Jörg Enderlein, Shimon Weiss

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

34 Scopus citations

Abstract

Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

Original language	English
Title of host publication	Nano-Biotechnology for Biomedical and Diagnostic Research
Editors	Eran Zahavy, Shmuel Yitzhaki, Arie Ordentlich, Avigdor Shafferman
Pages	17-21
Number of pages	5
DOIs	https://doi.org/10.1007/978-94-007-2555-3-2
State	Published - 2011
Externally published	Yes

Publication series

Name	Advances in Experimental Medicine and Biology
Volume	733
ISSN (Print)	0065-2598

Bibliographical note

Funding Information:
This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). Jörg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).

Funding

This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). Jörg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).

Funders	Funder number
National Institutes of Health	1R01GM086197
National Institute of Biomedical Imaging and Bioengineering	R01EB000312
Human Frontier Science Program	RGP46/2006
Deutsche Forschungsgemeinschaft	DE 1591/1 1
Bundesministerium für Bildung und Forschung	FKZ 13N9236

Keywords

Correlation function
Statistical analysis
Superresolution

Access to Document

10.1007/978-94-007-2555-3-2

Cite this

Dertinger, T., Colyer, R., Vogel, R., Heilemann, M., Sauer, M., Enderlein, J., & Weiss, S. (2011). Superresolution optical fluctuation imaging (SOFI). In E. Zahavy, S. Yitzhaki, A. Ordentlich, & A. Shafferman (Eds.), Nano-Biotechnology for Biomedical and Diagnostic Research (pp. 17-21). (Advances in Experimental Medicine and Biology; Vol. 733). https://doi.org/10.1007/978-94-007-2555-3-2

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title = "Superresolution optical fluctuation imaging (SOFI)",

abstract = "Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed {"}superresolution optical fluctuation imaging{"} (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.",

keywords = "Correlation function, Statistical analysis, Superresolution",

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note = "Funding Information: This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). J{\"o}rg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).",

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doi = "10.1007/978-94-007-2555-3-2",

language = "אנגלית",

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Dertinger, T, Colyer, R, Vogel, R, Heilemann, M, Sauer, M, Enderlein, J & Weiss, S 2011, Superresolution optical fluctuation imaging (SOFI). in E Zahavy, S Yitzhaki, A Ordentlich & A Shafferman (eds), Nano-Biotechnology for Biomedical and Diagnostic Research. Advances in Experimental Medicine and Biology, vol. 733, pp. 17-21. https://doi.org/10.1007/978-94-007-2555-3-2

Superresolution optical fluctuation imaging (SOFI). / Dertinger, Thomas; Colyer, Ryan; Vogel, Robert et al.
Nano-Biotechnology for Biomedical and Diagnostic Research. ed. / Eran Zahavy; Shmuel Yitzhaki; Arie Ordentlich; Avigdor Shafferman. 2011. p. 17-21 (Advances in Experimental Medicine and Biology; Vol. 733).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Superresolution optical fluctuation imaging (SOFI)

Abstract

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Keywords

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