Superresolution optical fluctuation imaging (SOFI)

Thomas Dertinger, Ryan Colyer, Robert Vogel, Mike Heilemann, Markus Sauer, Jörg Enderlein, Shimon Weiss

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

26 Scopus citations


Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

Original languageEnglish
Title of host publicationNano-Biotechnology for Biomedical and Diagnostic Research
EditorsEran Zahavy, Shmuel Yitzhaki, Arie Ordentlich, Avigdor Shafferman
Number of pages5
StatePublished - 2011
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)0065-2598

Bibliographical note

Funding Information:
This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). Jörg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).


  • Correlation function
  • Statistical analysis
  • Superresolution


Dive into the research topics of 'Superresolution optical fluctuation imaging (SOFI)'. Together they form a unique fingerprint.

Cite this