Abstract
Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of sugarbeet petioles. The transgenic plants were subjected to treatments known to cause oxidative stress, such as the herbicide methyl viologen and a natural photosensitizer toxin produced by the fungus Cercospora beticola, namely cercosporin. The transgenic plants exhibited increased tolerance to methyl viologen, to pure cercosporin, as well as to leaf infection with the fungus C. beticola.
| Original language | English |
|---|---|
| Pages (from-to) | 225-233 |
| Number of pages | 9 |
| Journal | Transgenic Research |
| Volume | 13 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2004 |
Bibliographical note
Funding Information:This work was supported by funds from the General Secretariat for Research and Technology (Greek Ministry of Development). We are grateful to Dr Skarakis of the Greek Sugar Industry Co. for providing the sugarbeet line 028 and CAMBIA for supplying the LBA4404 strain of Agrobacterium tumefaciens.
Keywords
- Agrobacterium tumefaciens
- Beta vulgaris L.
- cercospora
- disease resistance
- oxidative stress
- superoxide dismutase