Study of the structure of the transition metal-binding site of concanavalin A by extended X-ray absorption fine-structure spectroscopy

A. Joseph Kalb; E. A. Stern; S. M. Heald

doi:10.1016/0022-2836(79)90449-2

Study of the structure of the transition metal-binding site of concanavalin A by extended X-ray absorption fine-structure spectroscopy

A. Joseph Kalb, E. A. Stern, S. M. Heald

Research output: Contribution to journal › Article › peer-review

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Abstract

We have measured the extended X-ray absorption fine-structure spectra of Zn-concanavalin A and of Zn,Ca-concanavalin A on the high-energy side of the K absorption edge of Zn. Analysis of the spectra shows that, in Zn-concanavalin A, the Zn is hexaco-ordinate with a mean metal-to-ligand distance of 2.06 ± 0.l Å. Formation of Zn,Ca-concanavalin A by binding of Ca²⁺ at the calcium-specific site is accompanied by a decrease of the co-ordination number of the Zn from six to five with virtually no change in metal-to-ligand distance (2.02 ± 0.1 Å). The geometry of the hexaco-ordinate and pentaco-ordinate complexes and the mechanism of their interconversion are discussed.

Original language	English
Pages (from-to)	501-506
Number of pages	6
Journal	Journal of Molecular Biology
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/0022-2836(79)90449-2
State	Published - 5 Dec 1979
Externally published	Yes

Bibliographical note

Funding Information:
Thr help of the staff of the Stanford Sytlchroton Radiation Laboratory (SSRL) is deeply appreciated. The SSRL is support,ed by National Science Foundation grant no. DMR-73. 07692 in co-operationwith the Stanford Linear Accelerator Center and the U.S. Department of Energ-. The research of two of us (E. A. S. and S. M. H.) was slIpported in part by SSF grant no. PCH 76.21849 AOl.

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title = "Study of the structure of the transition metal-binding site of concanavalin A by extended X-ray absorption fine-structure spectroscopy",

abstract = "We have measured the extended X-ray absorption fine-structure spectra of Zn-concanavalin A and of Zn,Ca-concanavalin A on the high-energy side of the K absorption edge of Zn. Analysis of the spectra shows that, in Zn-concanavalin A, the Zn is hexaco-ordinate with a mean metal-to-ligand distance of 2.06 ± 0.l {\AA}. Formation of Zn,Ca-concanavalin A by binding of Ca2+ at the calcium-specific site is accompanied by a decrease of the co-ordination number of the Zn from six to five with virtually no change in metal-to-ligand distance (2.02 ± 0.1 {\AA}). The geometry of the hexaco-ordinate and pentaco-ordinate complexes and the mechanism of their interconversion are discussed.",

author = "Kalb, {A. Joseph} and Stern, {E. A.} and Heald, {S. M.}",

note = "Funding Information: Thr help of the staff of the Stanford Sytlchroton Radiation Laboratory (SSRL) is deeply appreciated. The SSRL is support,ed by National Science Foundation grant no. DMR-73. 07692 in co-operationwith the Stanford Linear Accelerator Center and the U.S. Department of Energ-. The research of two of us (E. A. S. and S. M. H.) was slIpported in part by SSF grant no. PCH 76.21849 AOl.",

year = "1979",

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doi = "10.1016/0022-2836(79)90449-2",

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AU - Kalb, A. Joseph

AU - Stern, E. A.

AU - Heald, S. M.

N1 - Funding Information: Thr help of the staff of the Stanford Sytlchroton Radiation Laboratory (SSRL) is deeply appreciated. The SSRL is support,ed by National Science Foundation grant no. DMR-73. 07692 in co-operationwith the Stanford Linear Accelerator Center and the U.S. Department of Energ-. The research of two of us (E. A. S. and S. M. H.) was slIpported in part by SSF grant no. PCH 76.21849 AOl.

PY - 1979/12/5

Y1 - 1979/12/5

N2 - We have measured the extended X-ray absorption fine-structure spectra of Zn-concanavalin A and of Zn,Ca-concanavalin A on the high-energy side of the K absorption edge of Zn. Analysis of the spectra shows that, in Zn-concanavalin A, the Zn is hexaco-ordinate with a mean metal-to-ligand distance of 2.06 ± 0.l Å. Formation of Zn,Ca-concanavalin A by binding of Ca2+ at the calcium-specific site is accompanied by a decrease of the co-ordination number of the Zn from six to five with virtually no change in metal-to-ligand distance (2.02 ± 0.1 Å). The geometry of the hexaco-ordinate and pentaco-ordinate complexes and the mechanism of their interconversion are discussed.

AB - We have measured the extended X-ray absorption fine-structure spectra of Zn-concanavalin A and of Zn,Ca-concanavalin A on the high-energy side of the K absorption edge of Zn. Analysis of the spectra shows that, in Zn-concanavalin A, the Zn is hexaco-ordinate with a mean metal-to-ligand distance of 2.06 ± 0.l Å. Formation of Zn,Ca-concanavalin A by binding of Ca2+ at the calcium-specific site is accompanied by a decrease of the co-ordination number of the Zn from six to five with virtually no change in metal-to-ligand distance (2.02 ± 0.1 Å). The geometry of the hexaco-ordinate and pentaco-ordinate complexes and the mechanism of their interconversion are discussed.

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Study of the structure of the transition metal-binding site of concanavalin A by extended X-ray absorption fine-structure spectroscopy

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