Structure–function studies of the G-domain from human gem, a novel small G-protein

Y. Opatowsky, Yehezkel Sasson, Isabella Shaked, Yvona Ward, Orna Chomsky-Hecht, ael Litvak, Zvi Selinger, Kathleen Kelly, Joel A. Hirsch

Research output: Contribution to journalArticlepeer-review

Abstract

Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique sequence features. We report here the crystallographic structure determination of the Gem G-domain in complex with nucleotide to 2.4 Å resolution. Although the basic Ras protein fold is maintained, the Gem switch regions emphatically differ from the Ras paradigm. Our ensuing biochemical characterization indicates that Gem G-domain markedly prefers GDP over GTP. Two known functions of Gem are distinctly affected by spatially separated clusters of mutations.
Original languageAmerican English
Pages (from-to)5959-5964
JournalFEBS Letters
Volume580
Issue number25
StatePublished - 2006

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