Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging

Kai Wen, Zhaolin Gao, Xiang Fang, Min Liu, Juanjuan Zheng, Ying Ma, Zeev Zalevsky, Peng Gao

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


This study presents a partially coherent illumination based (PCI-based) SIM apparatus for dual-modality (phase and fluorescent) microscopic imaging. The partially coherent illumination (PCI) is generated by placing a rotating diffuser on a monochromatic laser beam, which suppresses speckle noise in the dual-modality images and endows the apparatus with sound sectioning capability. With this system, label-free quantitative phase and super-resolved/sectioned fluorescent images can be obtained for the same sample. We have demonstrated the superiority of the system in phase imaging of transparent cells with high endogenous contrast and in a quantitative manner. In the meantime, we have also demonstrated fluorescent imaging of fluorescent beads, rat tail crosscut, wheat anther, and hibiscus pollen with super-resolution and optical sectioning. We envisage that the proposed method can be applied to many fields, including but not limited to biomedical, industrial, chemistry fields.

Original languageEnglish
Pages (from-to)33679-33693
Number of pages15
JournalOptics Express
Issue number21
StatePublished - 11 Oct 2021

Bibliographical note

Funding Information:
Funding. National Natural Science Foundation of China (62075177); Natural Science Foundation of Shaanxi Province (2020JM-193, 2020JQ-324, 2021JQ-184); Fundamental Research Funds for the Central Universities (JB210513, JC2112, XJS210503, XJS210504); Guangdong Basic and Applied Basic Research Foundation (2020A1515110590); State Key Laboratory of Transient Optics and Photonics; Key Laboratory of Image Processing and Pattern Recognition.

Publisher Copyright:
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.


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