TY - JOUR
T1 - Stimulation of human T cell colony growth by a lymphocyte colony enhancement factor derived from lymphocyte subpopulations
AU - Lenz, R.
AU - Alter, A.
AU - Radnay, J.
AU - Goldman, J.
AU - Cooper, O.
AU - Sredni, D.
AU - Rozenszajn, L. A.
PY - 1990
Y1 - 1990
N2 - Blood mononuclear cells (MNC) develop into T cell colonies when the cell are sensitized with PHA and seeded in a two-layer soft agar system. Conditioned medium (CM) derived from MNC enhanced lymphocyte colony formation when it was added to the culture system. CFU-TL appear to be stimulated into colony formation by molecules secreted by lymphocyte subpopulations contained in the seeded cells. In this study, human peripheral blood MNC were fractionated by a battery of techniques into adherent, E+, CD4+, CD8+, B and null cells. CM was prepared from each of the subpopulations and its effects on T cell colony growth assayed. All the lymphocyte subpopulations were found to generate lymphocyte colony enhancement factor (LCEF). After several purification procedures, CM prepared from CD4 and CD8+, displayed LCEF activity corresponding to proteins of molecular weight 30-40 and 100-140 kD.
AB - Blood mononuclear cells (MNC) develop into T cell colonies when the cell are sensitized with PHA and seeded in a two-layer soft agar system. Conditioned medium (CM) derived from MNC enhanced lymphocyte colony formation when it was added to the culture system. CFU-TL appear to be stimulated into colony formation by molecules secreted by lymphocyte subpopulations contained in the seeded cells. In this study, human peripheral blood MNC were fractionated by a battery of techniques into adherent, E+, CD4+, CD8+, B and null cells. CM was prepared from each of the subpopulations and its effects on T cell colony growth assayed. All the lymphocyte subpopulations were found to generate lymphocyte colony enhancement factor (LCEF). After several purification procedures, CM prepared from CD4 and CD8+, displayed LCEF activity corresponding to proteins of molecular weight 30-40 and 100-140 kD.
UR - http://www.scopus.com/inward/record.url?scp=0025076696&partnerID=8YFLogxK
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C2 - 1973528
AN - SCOPUS:0025076696
SN - 0254-7600
VL - 9
SP - 228
EP - 236
JO - Natural Immunity and Cell Growth Regulation
JF - Natural Immunity and Cell Growth Regulation
IS - 3
ER -