Stimulation of human peripheral lymphocytes and induction of interleukin 2 production by a lectin from the gonad of the sea hare, Aplysia fasciata

Danny Zipris; Nechama Gilboa-Garber

doi:10.1016/0145-305x(87)90039-5

Stimulation of human peripheral lymphocytes and induction of interleukin 2 production by a lectin from the gonad of the sea hare, Aplysia fasciata

Danny Zipris
, Nechama Gilboa-Garber

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The lectin from gonads of the sea hare, Aplysia fasciata, which reacts with D-galacturonic acid and D-galactose derivatives, was purified by affinity chromatography on Sepharose 4B. The purified lectin was shown to stimulate human peripheral blood lymphocytes and to induce interleukin 2 production like PHA. These activities were specifically inhibited by D-galactose and neutralized galacturonic acid (not by glucuronic acid). The rate of lymphocyte proliferation was similar at 72 and 96 hours in culture. The main stimulation was observed in the T lymphocyte population obtained by rosette formation with sheep red blood cells.

Original language	English
Pages (from-to)	501-511
Number of pages	11
Journal	Developmental and Comparative Immunology
Volume	11
Issue number	3
DOIs	https://doi.org/10.1016/0145-305x(87)90039-5
State	Published - 1987

Bibliographical note

Funding Information:
This report is a part of Ph.D. thesis of Mr. Danny Zipris. We wish to express our sincere thanks to Dr. Dina Fischer from the Weizmann Institute of Science for providing us CTLL 2 cells and for the helpful instructions concerning IL 2 assays. Special thanks to Drs. Abraham J. Susswein and Miriam Schwartz, Mr. Israel Ziv and Mrs. Silvia Markovich for supplying us the Aplysia. This research was supported by the health Sciences Research Center of the Department of Life Sciences, Bar-Ilan University, Israel.

Funding

This report is a part of Ph.D. thesis of Mr. Danny Zipris. We wish to express our sincere thanks to Dr. Dina Fischer from the Weizmann Institute of Science for providing us CTLL 2 cells and for the helpful instructions concerning IL 2 assays. Special thanks to Drs. Abraham J. Susswein and Miriam Schwartz, Mr. Israel Ziv and Mrs. Silvia Markovich for supplying us the Aplysia. This research was supported by the health Sciences Research Center of the Department of Life Sciences, Bar-Ilan University, Israel.

Funders
Bar-Ilan University

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10.1016/0145-305x(87)90039-5

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title = "Stimulation of human peripheral lymphocytes and induction of interleukin 2 production by a lectin from the gonad of the sea hare, Aplysia fasciata",

abstract = "The lectin from gonads of the sea hare, Aplysia fasciata, which reacts with D-galacturonic acid and D-galactose derivatives, was purified by affinity chromatography on Sepharose 4B. The purified lectin was shown to stimulate human peripheral blood lymphocytes and to induce interleukin 2 production like PHA. These activities were specifically inhibited by D-galactose and neutralized galacturonic acid (not by glucuronic acid). The rate of lymphocyte proliferation was similar at 72 and 96 hours in culture. The main stimulation was observed in the T lymphocyte population obtained by rosette formation with sheep red blood cells.",

author = "Danny Zipris and Nechama Gilboa-Garber",

note = "Funding Information: This report is a part of Ph.D. thesis of Mr. Danny Zipris. We wish to express our sincere thanks to Dr. Dina Fischer from the Weizmann Institute of Science for providing us CTLL 2 cells and for the helpful instructions concerning IL 2 assays. Special thanks to Drs. Abraham J. Susswein and Miriam Schwartz, Mr. Israel Ziv and Mrs. Silvia Markovich for supplying us the Aplysia. This research was supported by the health Sciences Research Center of the Department of Life Sciences, Bar-Ilan University, Israel.",

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Stimulation of human peripheral lymphocytes and induction of interleukin 2 production by a lectin from the gonad of the sea hare, Aplysia fasciata

Abstract

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