Abstract
The lectin from gonads of the sea hare, Aplysia fasciata, which reacts with D-galacturonic acid and D-galactose derivatives, was purified by affinity chromatography on Sepharose 4B. The purified lectin was shown to stimulate human peripheral blood lymphocytes and to induce interleukin 2 production like PHA. These activities were specifically inhibited by D-galactose and neutralized galacturonic acid (not by glucuronic acid). The rate of lymphocyte proliferation was similar at 72 and 96 hours in culture. The main stimulation was observed in the T lymphocyte population obtained by rosette formation with sheep red blood cells.
| Original language | English |
|---|---|
| Pages (from-to) | 501-511 |
| Number of pages | 11 |
| Journal | Developmental and Comparative Immunology |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1987 |
Bibliographical note
Funding Information:This report is a part of Ph.D. thesis of Mr. Danny Zipris. We wish to express our sincere thanks to Dr. Dina Fischer from the Weizmann Institute of Science for providing us CTLL 2 cells and for the helpful instructions concerning IL 2 assays. Special thanks to Drs. Abraham J. Susswein and Miriam Schwartz, Mr. Israel Ziv and Mrs. Silvia Markovich for supplying us the Aplysia. This research was supported by the health Sciences Research Center of the Department of Life Sciences, Bar-Ilan University, Israel.