Stable, compact, bright biofunctional quantum dots with improved peptide coating

Jianmin Xu; Piotr Ruchala; Yuval Ebenstain; J. Jack Li; Shimon Weiss

doi:10.1021/jp306453y

Stable, compact, bright biofunctional quantum dots with improved peptide coating

Jianmin Xu, Piotr Ruchala, Yuval Ebenstain, J. Jack Li, Shimon Weiss

University of California at Los Angeles

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

We developed a new peptide, natural phytochelatin (PC), which tightly binds to CdSe/ZnS quantum dots' (QDs) surfaces and renders them water-soluble. Coating QDs with this flexible and all-hydrophilic peptide offers high colloidal stability, adds only 0.8-0.9 nm to the radius of the particles (as compared to their original inorganic radius), preserves very high quantum yield (QY) in water, and affords facile bioconjugation with various functional groups. We demonstrate specific targeting (with minimal nonspecific binding) of such fluorescein-conjugated QDs to ScFv-fused mouse prion protein expressed in live N2A cells. We also demonstrated homogeneous in vivo biodistribution with no significant toxicity in live zebrafish.

Original language	English
Pages (from-to)	11370-11378
Number of pages	9
Journal	Journal of Physical Chemistry B
Volume	116
Issue number	36
DOIs	https://doi.org/10.1021/jp306453y
State	Published - 13 Sep 2012
Externally published	Yes

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abstract = "We developed a new peptide, natural phytochelatin (PC), which tightly binds to CdSe/ZnS quantum dots' (QDs) surfaces and renders them water-soluble. Coating QDs with this flexible and all-hydrophilic peptide offers high colloidal stability, adds only 0.8-0.9 nm to the radius of the particles (as compared to their original inorganic radius), preserves very high quantum yield (QY) in water, and affords facile bioconjugation with various functional groups. We demonstrate specific targeting (with minimal nonspecific binding) of such fluorescein-conjugated QDs to ScFv-fused mouse prion protein expressed in live N2A cells. We also demonstrated homogeneous in vivo biodistribution with no significant toxicity in live zebrafish.",

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AU - Xu, Jianmin

AU - Ruchala, Piotr

AU - Ebenstain, Yuval

AU - Li, J. Jack

AU - Weiss, Shimon

PY - 2012/9/13

Y1 - 2012/9/13

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AB - We developed a new peptide, natural phytochelatin (PC), which tightly binds to CdSe/ZnS quantum dots' (QDs) surfaces and renders them water-soluble. Coating QDs with this flexible and all-hydrophilic peptide offers high colloidal stability, adds only 0.8-0.9 nm to the radius of the particles (as compared to their original inorganic radius), preserves very high quantum yield (QY) in water, and affords facile bioconjugation with various functional groups. We demonstrate specific targeting (with minimal nonspecific binding) of such fluorescein-conjugated QDs to ScFv-fused mouse prion protein expressed in live N2A cells. We also demonstrated homogeneous in vivo biodistribution with no significant toxicity in live zebrafish.

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Stable, compact, bright biofunctional quantum dots with improved peptide coating

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