TY - JOUR
T1 - Sperm motility analyzer (SMA), a practical tool of motility and cell concentration determinations in artificial insemination centers
AU - Bartoov, B.
AU - Kalay, D.
AU - Mayevsky, A.
PY - 1981
Y1 - 1981
N2 - A description of a new instrument, which analyzed objectively sperm cell concentration and collective motility of a fresh semen sample of bull or ram, is reported. A display read-out of sperm cell concentration for an aliquot of the undiluted semen sample in the range from 200 × 106 to 4000 × 106 cell/ml with an accuracy of ± 5% is shown immediately by optical density units. A significant correlation coefficient (0.918, p < 0.01) was obtained between these units and cell concentrations, as determined in four A.I. centers in the U.S. and Canada. The intensity of the sperm collective motility is expressed as an objective index (SMI) which was significantly correlated to the fraction of the live motile cells in the semen sample. The SMI values were corrected for a possible error originating from the difference in sperm cell concentration by an empirical equation. Due to the accuracy, repeatability and objectivity of evaluating the semen quality and also the simplicity of operation, as well as the possibility of utilizing the measured semen sample for further processing, the Sperm Motility Analyzer (SMA) seems suitable as a standard tool for semen quality control in the A.I. centers.
AB - A description of a new instrument, which analyzed objectively sperm cell concentration and collective motility of a fresh semen sample of bull or ram, is reported. A display read-out of sperm cell concentration for an aliquot of the undiluted semen sample in the range from 200 × 106 to 4000 × 106 cell/ml with an accuracy of ± 5% is shown immediately by optical density units. A significant correlation coefficient (0.918, p < 0.01) was obtained between these units and cell concentrations, as determined in four A.I. centers in the U.S. and Canada. The intensity of the sperm collective motility is expressed as an objective index (SMI) which was significantly correlated to the fraction of the live motile cells in the semen sample. The SMI values were corrected for a possible error originating from the difference in sperm cell concentration by an empirical equation. Due to the accuracy, repeatability and objectivity of evaluating the semen quality and also the simplicity of operation, as well as the possibility of utilizing the measured semen sample for further processing, the Sperm Motility Analyzer (SMA) seems suitable as a standard tool for semen quality control in the A.I. centers.
UR - http://www.scopus.com/inward/record.url?scp=0000454287&partnerID=8YFLogxK
U2 - 10.1016/s0093-691x(81)80005-2
DO - 10.1016/s0093-691x(81)80005-2
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AN - SCOPUS:0000454287
SN - 0093-691X
VL - 15
SP - 173
EP - 182
JO - Theriogenology
JF - Theriogenology
IS - 2
ER -