Speckle random coding for 2D super resolving fluorescent microscopic imaging

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Abstract

In this manuscript we present a novel super resolving approach based upon projection of a random speckle pattern onto samples observed through a microscope. The projection of the speckle pattern is created by coherent illumination of the inspected pattern through a diffuser. Due to local interference of the coherent wave front with itself, a random speckle pattern is superimposed on the sample. This speckle pattern can be scanned over the object. A super-resolved image can be extracted from a temporal sequence of images by appropriate digital processing of the image stream. The resulting resolution is significantly higher than the diffraction limitation of the microscope objective. The new super-resolution method is demonstrated by application to fluorescence of biological samples.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalMicron
Volume38
Issue number2
DOIs
StatePublished - Feb 2007

Bibliographical note

Funding Information:
Javier Garcia acknowledges the support from the Spanish Ministry of Science and Education and the ministry of Science and Technology under the codes PR-2004-0543 and FIS2004-06947-C02-01.

Keywords

  • Fluorescence microscope
  • Numerical aperture
  • Speckle pattern

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