In this manuscript we present a novel super resolving approach based upon projection of a random speckle pattern onto samples observed through a microscope. The projection of the speckle pattern is created by coherent illumination of the inspected pattern through a diffuser. Due to local interference of the coherent wave front with itself, a random speckle pattern is superimposed on the sample. This speckle pattern can be scanned over the object. A super-resolved image can be extracted from a temporal sequence of images by appropriate digital processing of the image stream. The resulting resolution is significantly higher than the diffraction limitation of the microscope objective. The new super-resolution method is demonstrated by application to fluorescence of biological samples.
Bibliographical noteFunding Information:
Javier Garcia acknowledges the support from the Spanish Ministry of Science and Education and the ministry of Science and Technology under the codes PR-2004-0543 and FIS2004-06947-C02-01.
- Fluorescence microscope
- Numerical aperture
- Speckle pattern