Specificity of photosensitizer accumulation in undifferentiated versus differentiated colon carcinoma cells

G. Siboni; I. Amit-Patito; E. Weizman; M. Waintraub-Porat; H. Weitman; B. Ehrenberg; Z. Malik

doi:10.1016/s0304-3835(03)00207-6

Specificity of photosensitizer accumulation in undifferentiated versus differentiated colon carcinoma cells

G. Siboni, I. Amit-Patito, E. Weizman, M. Waintraub-Porat, H. Weitman, B. Ehrenberg, Z. Malik

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The relationship between tumor cell differentiation and photosensitizer accumulation used in PDT is poorly defined. In the present work, specific cell differentiation of colon carcinoma CT26 cells induced by sodium butyrate was manifested by morphological changes, proliferation and protein expression and was correlated with the accumulation of endogenous and exogenous photosensitizes. Reduced accumulation of the endogenous protoporphyrin IX and the exogenous hypericin and MC540 was detected in differentiated cells. In contrast, a differentiation-dependent increase was measured with TPPS₄, TMPyP, the pheophorbides (C5, C6, C12), HypS₄ and helianthrone. In conclusion, PpIX, Hypericin and MC540 show specific binding and accumulation in poorly differentiated tumors, giving these tumors tissue-specific advantage in photo-diagnostic PDT applications.

Original language	English
Pages (from-to)	57-64
Number of pages	8
Journal	Cancer Letters
Volume	196
Issue number	1
DOIs	https://doi.org/10.1016/s0304-3835(03)00207-6
State	Published - 30 Jun 2003

Bibliographical note

Funding Information:
We wish to acknowledge the support of the United States–Israel Binational Science Foundation (BSF Grant No. 9800364), Jerusalem, Israel (to B.E.). Z. Malik and B. Ehrenberg acknowledge the support of the Michael David Falk Chair in Laser Phototherapy. We also thank Prof. Y. Mazur and Dr D. Freeman from the Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel, for synthesizing the hypericin (Hyp), hypericin tetrasulfonic acid (HypS 4 ) and tetrahydroxyhelianthrone (Hel).

Funding

We wish to acknowledge the support of the United States–Israel Binational Science Foundation (BSF Grant No. 9800364), Jerusalem, Israel (to B.E.). Z. Malik and B. Ehrenberg acknowledge the support of the Michael David Falk Chair in Laser Phototherapy. We also thank Prof. Y. Mazur and Dr D. Freeman from the Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel, for synthesizing the hypericin (Hyp), hypericin tetrasulfonic acid (HypS 4 ) and tetrahydroxyhelianthrone (Hel).

Funders	Funder number
Bloom's Syndrome Foundation	9800364
United States-Israel Binational Science Foundation

Keywords

Alkaline phosphatase
Differentiation
Photosensitizer
PpIX
p21
p27

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/s0304-3835(03)00207-6

Cite this

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title = "Specificity of photosensitizer accumulation in undifferentiated versus differentiated colon carcinoma cells",

abstract = "The relationship between tumor cell differentiation and photosensitizer accumulation used in PDT is poorly defined. In the present work, specific cell differentiation of colon carcinoma CT26 cells induced by sodium butyrate was manifested by morphological changes, proliferation and protein expression and was correlated with the accumulation of endogenous and exogenous photosensitizes. Reduced accumulation of the endogenous protoporphyrin IX and the exogenous hypericin and MC540 was detected in differentiated cells. In contrast, a differentiation-dependent increase was measured with TPPS4, TMPyP, the pheophorbides (C5, C6, C12), HypS4 and helianthrone. In conclusion, PpIX, Hypericin and MC540 show specific binding and accumulation in poorly differentiated tumors, giving these tumors tissue-specific advantage in photo-diagnostic PDT applications.",

keywords = "Alkaline phosphatase, Differentiation, Photosensitizer, PpIX, p21, p27",

author = "G. Siboni and I. Amit-Patito and E. Weizman and M. Waintraub-Porat and H. Weitman and B. Ehrenberg and Z. Malik",

note = "Funding Information: We wish to acknowledge the support of the United States–Israel Binational Science Foundation (BSF Grant No. 9800364), Jerusalem, Israel (to B.E.). Z. Malik and B. Ehrenberg acknowledge the support of the Michael David Falk Chair in Laser Phototherapy. We also thank Prof. Y. Mazur and Dr D. Freeman from the Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel, for synthesizing the hypericin (Hyp), hypericin tetrasulfonic acid (HypS 4 ) and tetrahydroxyhelianthrone (Hel).",

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AU - Siboni, G.

AU - Amit-Patito, I.

AU - Weizman, E.

AU - Waintraub-Porat, M.

AU - Weitman, H.

AU - Ehrenberg, B.

AU - Malik, Z.

N1 - Funding Information: We wish to acknowledge the support of the United States–Israel Binational Science Foundation (BSF Grant No. 9800364), Jerusalem, Israel (to B.E.). Z. Malik and B. Ehrenberg acknowledge the support of the Michael David Falk Chair in Laser Phototherapy. We also thank Prof. Y. Mazur and Dr D. Freeman from the Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel, for synthesizing the hypericin (Hyp), hypericin tetrasulfonic acid (HypS 4 ) and tetrahydroxyhelianthrone (Hel).

PY - 2003/6/30

Y1 - 2003/6/30

N2 - The relationship between tumor cell differentiation and photosensitizer accumulation used in PDT is poorly defined. In the present work, specific cell differentiation of colon carcinoma CT26 cells induced by sodium butyrate was manifested by morphological changes, proliferation and protein expression and was correlated with the accumulation of endogenous and exogenous photosensitizes. Reduced accumulation of the endogenous protoporphyrin IX and the exogenous hypericin and MC540 was detected in differentiated cells. In contrast, a differentiation-dependent increase was measured with TPPS4, TMPyP, the pheophorbides (C5, C6, C12), HypS4 and helianthrone. In conclusion, PpIX, Hypericin and MC540 show specific binding and accumulation in poorly differentiated tumors, giving these tumors tissue-specific advantage in photo-diagnostic PDT applications.

AB - The relationship between tumor cell differentiation and photosensitizer accumulation used in PDT is poorly defined. In the present work, specific cell differentiation of colon carcinoma CT26 cells induced by sodium butyrate was manifested by morphological changes, proliferation and protein expression and was correlated with the accumulation of endogenous and exogenous photosensitizes. Reduced accumulation of the endogenous protoporphyrin IX and the exogenous hypericin and MC540 was detected in differentiated cells. In contrast, a differentiation-dependent increase was measured with TPPS4, TMPyP, the pheophorbides (C5, C6, C12), HypS4 and helianthrone. In conclusion, PpIX, Hypericin and MC540 show specific binding and accumulation in poorly differentiated tumors, giving these tumors tissue-specific advantage in photo-diagnostic PDT applications.

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Specificity of photosensitizer accumulation in undifferentiated versus differentiated colon carcinoma cells

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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