Specific activity of carp antisera against β2-microglobulin (β2m) and evidence for a β2m homologue in carp (Cyprinus carpio)

A. Shalev; R. R. Caspi; L. Lögdberg; L. Björck; S. Segal; R. R. Avtalion

doi:10.1016/0145-305x(84)90096-x

Specific activity of carp antisera against β₂-microglobulin (β₂m) and evidence for a β₂m homologue in carp (Cyprinus carpio)

A. Shalev
, R. R. Caspi
, L. Lögdberg
, L. Björck
, S. Segal
, R. R. Avtalion

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Antisera against rabbit and human β₂-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β₂m, and detected β₂m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β₂m to distinguish between mouse and rat β₂m's suggested that the carp itself carries β₂m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β₂m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β₂m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β₂m.

Original language	English
Pages (from-to)	639-648
Number of pages	10
Journal	Developmental and Comparative Immunology
Volume	8
Issue number	3
DOIs	https://doi.org/10.1016/0145-305x(84)90096-x
State	Published - 1984

Bibliographical note

Funding Information:
This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.

Funding

This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.

Funders	Funder number
KGSS
Israel Academy of Sciences and Humanities
Medicinska Forskningsrådet	6456

Access to Document

10.1016/0145-305x(84)90096-x

Cite this

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title = "Specific activity of carp antisera against β2-microglobulin (β2m) and evidence for a β2m homologue in carp (Cyprinus carpio)",

abstract = "Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.",

author = "A. Shalev and Caspi, \{R. R.\} and L. L{\"o}gdberg and L. Bj{\"o}rck and S. Segal and Avtalion, \{R. R.\}",

note = "Funding Information: This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog \textasciitilde{}2m antiserum and the horse anti-human B2m antiserum.",

year = "1984",

doi = "10.1016/0145-305x(84)90096-x",

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AU - Caspi, R. R.

AU - Lögdberg, L.

AU - Björck, L.

AU - Segal, S.

AU - Avtalion, R. R.

N1 - Funding Information: This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.

PY - 1984

Y1 - 1984

N2 - Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.

AB - Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.

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