Specific activity of carp antisera against β2-microglobulin (β2m) and evidence for a β2m homologue in carp (Cyprinus carpio)

A. Shalev, R. R. Caspi, L. Lögdberg, L. Björck, S. Segal, R. R. Avtalion

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.

Original languageEnglish
Pages (from-to)639-648
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume8
Issue number3
DOIs
StatePublished - 1984

Bibliographical note

Funding Information:
This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.

Funding

This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.

FundersFunder number
KGSS
Israel Academy of Sciences and Humanities
Medicinska Forskningsrådet6456

    Fingerprint

    Dive into the research topics of 'Specific activity of carp antisera against β2-microglobulin (β2m) and evidence for a β2m homologue in carp (Cyprinus carpio)'. Together they form a unique fingerprint.

    Cite this