Abstract
Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.
Original language | English |
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Pages (from-to) | 639-648 |
Number of pages | 10 |
Journal | Developmental and Comparative Immunology |
Volume | 8 |
Issue number | 3 |
DOIs | |
State | Published - 1984 |
Bibliographical note
Funding Information:This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.
Funding
This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.
Funders | Funder number |
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KGSS | |
Israel Academy of Sciences and Humanities | |
Medicinska Forskningsrådet | 6456 |