Spatial-temporal correlations in the speckle pattern for the characterization of cellular motion within a 3D object

Y Weil, Y Shafran, M Sobolev, L Afrimzon, N Zurgil, M Deutsch, Z Schiffer

Research output: Contribution to journalArticlepeer-review

Abstract

Dynamic light scattering analysis has been demonstrated recently to be a promising tool for the assessment of structural changes taking place inside opaque tissue samples. Specifically, quantification of velocity and direction of cellular motion inside spheroids and organoids has attracted much attention as a potent indicator in personalized therapy research. Here, we propose a method for the quantitative extraction of cellular motion, velocity, and direction, by applying a concept of speckle spatial-temporal correlation dynamics. Numerical simulations and experimental results obtained on phantom and biological spheroids are presented. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
Original languageEnglish
Pages (from-to)1974-1991
Number of pages18
JournalBiomedical Optics Express
Volume14
Issue number5
DOIs
StatePublished - 1 May 2023

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