Spatial organization and early signaling of the B-cell receptor in CLL

Yamit Shorer Arbel, Yotam Bronstein, Tali Dadosh, Talia Kamdjou, Shlomo Tsuriel, Mika Shapiro, Ben Zion Katz, Yair Herishanu

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Most chronic lymphocytic leukemia (CLL) clones express B-cell receptors (BcR) of both IgM/IgD isotypes; however, 5%–10% of CLL cases express isotype-switched immunoglobulin G (IgG). The early signaling and spatial patterning of the various BcRs at steady state and after activation are still fully unresolved. Herein, we show higher expression of the BcR signalosome elements and a more robust constitutive cell-intrinsic proximal BcR signaling in CLL with unmutated IGHV expressing IgM isotype (IgM U-CLL), compared with IGHV-mutated CLL (M-CLL) expressing either IgM or IgG isotypes. IgM in U-CLL is frequently located in the membrane plane in polarized patches, occasionally in caps, and sometimes inside the cells. Among M-CLL, IgM is scattered laterally in the membrane plane in a similar pattern as seen in normal B cells, whereas IgG is dispersed around the cell membrane in smaller clusters than in IgM U-CLL. Upon BcR engagement, both IgG and IgM expressing M-CLL showed attenuated signaling and only slight spatial reorganization dynamics of BcR microclusters and internalization, compared with the extensive reorganization and internalization of the BcR in IgM expressing U-CLL. The global gene signature of IgG M-CLL was closely related to that of IgM M-CLL rather than IgM U-CLL. Overall, we report fundamental differences in the basal composition, biochemical status, and spatial organization of the BcR in the three examined immunogenetic CLL subtypes that correlate with their clinical behavior. On the basis of our findings, IgG class-switched M-CLL likely represents the same disease as IgM M-CLL rather than a different biological and/or clinical entity.

Original languageEnglish
Article number953660
JournalFrontiers in Immunology
StatePublished - 9 Aug 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2022 Shorer Arbel, Bronstein, Dadosh, Kamdjou, Tsuriel, Shapiro, Katz and Herishanu.


  • BCR signaling
  • CLL (chronic lymphocytic leukemia)
  • d-STORM
  • IgG
  • IGHV mutational status
  • IgM


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