Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics

Olga Grinberg; Aharon Gedanken; Chitta Ranjan Patra; Sujata Patra; Priyabrata Mukherjee; Debabrata Mukhopadhyay

doi:10.1016/j.actbio.2009.05.003

Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics

Olga Grinberg
, Aharon Gedanken
, Chitta Ranjan Patra
, Sujata Patra
, Priyabrata Mukherjee
, Debabrata Mukhopadhyay

Nanotechnology Research Center
Mayo Clinic College of Medicine and Science

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [³H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.

Original language	English
Pages (from-to)	3031-3037
Number of pages	7
Journal	Acta Biomaterialia
Volume	5
Issue number	8
DOIs	https://doi.org/10.1016/j.actbio.2009.05.003
State	Published - Oct 2009
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

BSA microspheres
Drug delivery
Gemcitabine
Renal cancer 786-O cells
Sonochemistry

Access to Document

10.1016/j.actbio.2009.05.003

Cite this

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title = "Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics",

abstract = "This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [3H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.",

keywords = "BSA microspheres, Drug delivery, Gemcitabine, Renal cancer 786-O cells, Sonochemistry",

author = "Olga Grinberg and Aharon Gedanken and Patra, \{Chitta Ranjan\} and Sujata Patra and Priyabrata Mukherjee and Debabrata Mukhopadhyay",

year = "2009",

month = oct,

doi = "10.1016/j.actbio.2009.05.003",

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AU - Grinberg, Olga

AU - Gedanken, Aharon

AU - Patra, Chitta Ranjan

AU - Patra, Sujata

AU - Mukherjee, Priyabrata

AU - Mukhopadhyay, Debabrata

PY - 2009/10

Y1 - 2009/10

N2 - This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [3H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.

AB - This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [3H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.

KW - BSA microspheres

KW - Drug delivery

KW - Gemcitabine

KW - Renal cancer 786-O cells

KW - Sonochemistry

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DO - 10.1016/j.actbio.2009.05.003

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AN - SCOPUS:70349202500

SN - 1742-7061

VL - 5

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JO - Acta Biomaterialia

JF - Acta Biomaterialia

IS - 8

ER -

Sonochemically prepared BSA microspheres containing Gemcitabine, and their potential application in renal cancer therapeutics

Abstract

UN SDGs

Keywords

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