Abstract
This report demonstrates the formation and characterization of sonochemically prepared bovine serum albumin (BSA)-Gemzar (Gemcitabine) microspheres and shows their increased anticancer activity compared to pristine Gemzar. The amount of loaded Gemzar was determined by light absorption measurements. The BSA-Gemzar composite was analyzed and characterized by optical microscopy and scanning electron microscopy. The release kinetics of Gemzar from the proteinaceous microspheres was tested. The BSA-Gemzar composite was examined for its anticancer activity (in vitro) in renal cancer cells (RCC, 786-O cells) using [3H]thymidine incorporation assays. It was found that the influence of the Gemzar-loaded microspheres on the cancer cells was significantly greater than that of an equimolar concentration of pristine Gemzar.
Original language | English |
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Pages (from-to) | 3031-3037 |
Number of pages | 7 |
Journal | Acta Biomaterialia |
Volume | 5 |
Issue number | 8 |
DOIs | |
State | Published - Oct 2009 |
Externally published | Yes |
Keywords
- BSA microspheres
- Drug delivery
- Gemcitabine
- Renal cancer 786-O cells
- Sonochemistry