Sonochemically modified ovalbumin enhances enantioenrichment of some amino acids

Amruta Mutalikdesai, Molhm Nassir, Abed Saady, Alfred Hassner, Aharon Gedanken

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As part of our efforts to develop a new method for chiral resolution of amino acids with sonochemically modified proteins, we present result that indicates how ovalbumin microspheres (OAMS) interact specifically with L-amino acids from a racemate in solution, leaving an excess of D-enantiomer in the permeate solution. Among different amino acids that interacted with the OAMS, tryptophan (Trp) was the most successfully resolved with 65% enantiomeric excess. A control experiment with native ovalbumin in solution did not show any chiral resolution of amino acids. Interestingly, when the OAMS were pretreated with racemic lysine (Lys) solution and then used for resolution of tryptophan the enantiomeric enrichment of D-tryptophan was raised to 98%. This unanticipated positive effect is discussed in terms of the structural correlation between Trp and Lys, which is less apparent in other amino acids such as phenylalanine.

Original languageEnglish
Article number104603
JournalUltrasonics Sonochemistry
StatePublished - Nov 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier B.V.


  • Microspheres and Chiral separation
  • Ovalbumin
  • Sonication


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