Some electrophysiological properties of cultured rat cerebral cortical neurons dissociated from fetuses at various gestational ages

Neurons from dissociated cerebral cortex of fetal rat of different gestational ages were grown in culture for up to 4 weeks. Studies of membrane and action potentials, input resistance, neuron size and neurite outgrowth showed that neurons from 7-day fetuses develop rapidly both electrophysiologically and morphologically, but are maintained for only about 2 weeks. In contrast, neurons from 14 to 17 day fetuses mature slowly, but can be maintained for at least 4 weeks. Neurons from both young and old fetuses show an increase in resting Em with age, the maximum value of -60 to -65 mV being attained by about 2 weeks. While neurons can be maintained in serum-free medium, their membrane electrical properties deteriorate with time. Thus, appropriate development of neurons in culture depends upon both the age of the starting tissue and the presence of adequate, but still undefined factors found in animal serum for at least the first several days in culture.