Somatic embryo proliferation in Commiphora wightii and evidence for guggulsterone production in culture

Sandeep Kumar, Meeta Mathur, A. K. Jain, K. G. Ramawat

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A method for obtaining somatic embryos consistently in Commiphora wightii (Arnott.) Bhandari has been developed. Somatic embryos when sub-cultured on modified MS medium containing ABA (10 μg L-1) produced more embryos directly by meristematic activity of the epidermal and subepidermal cells at the hypocotyl region or also from the root tip region. Embryo proliferation was also observed on auxin-cytokinin free modified MS medium supplemented with different concentrations of activated charcoal, abscisic acid, mannitol and sucrose. Modified MS hormone-free medium containing activated charcoal 0.5 g L-1 and sucrose 10 g L-1 was most suitable for multiplication and maturation of embryos. Embryo formation was also recorded from callus produced by earlier formed somatic embryos (indirect). Embryos obtained from various maturation treatments when desiccated under laminar airflow bench for 5-9 d showed maximum germination percentage. Guggulsterone-E and -Z contents of in vitro embryonic cultures were 1/4 - and 1/2-fold of that found in zygotic embryos, but several fold higher than callus cultures, therefore, hold promise for the production of bioactive guggulsterones.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalIndian Journal of Biotechnology
Volume5
Issue number2
StatePublished - Apr 2006
Externally publishedYes

Keywords

  • Antihyperlipidemia
  • Callus cultures
  • Commiphora wightii
  • Guggulsterones
  • Somatic embryogenesis

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