Abstract
Biofilms are accumulations of microorganisms embedded in extracellular matrices that protect against external factors and stressful environments. Cyanobacterial biofilms are ubiquitous and have potential for treatment of wastewater and sustainable production of biofuels. But the underlying mechanisms regulating cyanobacterial biofilm formation are unclear. Here, we report the solution NMR structure of a protein, Se0862, conserved across diverse cyanobacterial species and involved in regulation of biofilm formation in the cyanobacterium Synechococcus elongatus PCC 7942. Se0862 is a class α+β protein with ααββββαα topology and roll architecture, consisting of a four-stranded β-sheet that is flanked by four α-helices on one side. Conserved surface residues constitute a hydrophobic pocket and charged regions that are likely also present in Se0862 orthologs.
Original language | English |
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Pages (from-to) | 2274-2280 |
Number of pages | 7 |
Journal | Protein Science |
Volume | 29 |
Issue number | 11 |
DOIs | |
State | Published - 1 Nov 2020 |
Bibliographical note
Publisher Copyright:© 2020 The Protein Society
Funding
This research was supported by grants from Air Force Office of Scientific Research (FA9550‐17‐1‐0447) and NSF‐CREST: Center for Cellular and Biomolecular Machines at the University of California, Merced (NSF‐HRD‐1547848). Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01‐GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. This research was supported by grants from Air Force Office of Scientific Research (FA9550-17-1-0447) and NSF-CREST: Center for Cellular and Biomolecular Machines at the University of California, Merced (NSF-HRD-1547848). Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases.
Funders | Funder number |
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NSF-CREST | |
NSF‐CREST | NSF‐HRD‐1547848 |
National Institutes of Health | |
National Institute of General Medical Sciences | R01GM129325 |
National Institute of Allergy and Infectious Diseases | |
Air Force Office of Scientific Research | FA9550‐17‐1‐0447 |
Keywords
- NMR spectroscopy
- S. elongatus PCC 7942
- biofilm
- cyanobacteria
- protein structure