Solid-phase radioimmunoassay for the measurement of surface antigens expressed on intact lymphocytes

Shoshana Savion; Yedida Sharabi; Jacob Shoham

doi:10.1016/0022-1759(87)90104-9

Solid-phase radioimmunoassay for the measurement of surface antigens expressed on intact lymphocytes

Shoshana Savion, Yedida Sharabi, Jacob Shoham

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A solid-phase radioimmunoassay was developed for the measurement of lymphocyte surface antigens. The assay was performed in microplates, using cells that were initially fixed to the wells by air drying. The method was used for the measurement of Thy-1, Lyt-1,2,3, IL-2-R, H-2K^b and DR antigens on the surface of mouse thymus, spleen and bone marrow cells, mouse cell lines CTLL, EL-4 and DA-1 and human thymocytes and consisted of sequential incubations with rat or mouse monoclonal antibodies directed against the above antigens, rabbit anti-rat or goat anti-mouse IgG and ¹²⁵I-protein A. The assay permits the processing of large numbers of samples, is easy to perform, reliable and highly specific.

Original language	English
Pages (from-to)	49-56
Number of pages	8
Journal	Journal of Immunological Methods
Volume	97
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(87)90104-9
State	Published - 26 Feb 1987

Bibliographical note

Funding Information:
This work was supported by a grant from the Istituto Farmacologico Serono, Rome, Italy. We thank Dr. M. Osband, Boston, MA, for fruitful discussions.

Funding

This work was supported by a grant from the Istituto Farmacologico Serono, Rome, Italy. We thank Dr. M. Osband, Boston, MA, for fruitful discussions.

Funders	Funder number
Istituto Farmacologico Serono

Keywords

Binding test
Cell surface antigen
Protein A
Radioimmunoassay
solid-phase

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10.1016/0022-1759(87)90104-9

Cite this

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title = "Solid-phase radioimmunoassay for the measurement of surface antigens expressed on intact lymphocytes",

abstract = "A solid-phase radioimmunoassay was developed for the measurement of lymphocyte surface antigens. The assay was performed in microplates, using cells that were initially fixed to the wells by air drying. The method was used for the measurement of Thy-1, Lyt-1,2,3, IL-2-R, H-2Kb and DR antigens on the surface of mouse thymus, spleen and bone marrow cells, mouse cell lines CTLL, EL-4 and DA-1 and human thymocytes and consisted of sequential incubations with rat or mouse monoclonal antibodies directed against the above antigens, rabbit anti-rat or goat anti-mouse IgG and 125I-protein A. The assay permits the processing of large numbers of samples, is easy to perform, reliable and highly specific.",

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AU - Shoham, Jacob

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Solid-phase radioimmunoassay for the measurement of surface antigens expressed on intact lymphocytes

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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