Sleep increases chromosome dynamics to enable reduction of accumulating DNA damage in single neurons

D. Zada; I. Bronshtein; T. Lerer-Goldshtein; Y. Garini; L. Appelbaum

doi:10.1038/s41467-019-08806-w

Sleep increases chromosome dynamics to enable reduction of accumulating DNA damage in single neurons

D. Zada, I. Bronshtein, T. Lerer-Goldshtein, Y. Garini, L. Appelbaum

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Sleep is essential to all animals with a nervous system. Nevertheless, the core cellular function of sleep is unknown, and there is no conserved molecular marker to define sleep across phylogeny. Time-lapse imaging of chromosomal markers in single cells of live zebrafish revealed that sleep increases chromosome dynamics in individual neurons but not in two other cell types. Manipulation of sleep, chromosome dynamics, neuronal activity, and DNA double-strand breaks (DSBs) showed that chromosome dynamics are low and the number of DSBs accumulates during wakefulness. In turn, sleep increases chromosome dynamics, which are necessary to reduce the amount of DSBs. These results establish chromosome dynamics as a potential marker to define single sleeping cells, and propose that the restorative function of sleep is nuclear maintenance.

Original language	English
Article number	895
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-08806-w
State	Published - 5 Mar 2019

Bibliographical note

Publisher Copyright:
© 2019, The Author(s).

Funding

The authors thank the members of the Appelbaum lab for technical assistance, Noa Alon for assistance in designing the schematic illustrations, and Luis de Lecea, David Klein, German Sumbre, Eran Meshorer, and Ofer Yizhar for helpful discussions and comments. The authors also thank Ms. Sharon Victor for assistance in editing the manuscript. This work was supported by the Israel Science Foundation (Grant no. 690/ 15 to L.A.) and by the US–Israel Binational Science Foundation (BSF, Grant no. 2017105 to L.A.).

Funders	Funder number
United States-Israel Binational Science Foundation	2017105
Israel Science Foundation	690/ 15

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Sleep increases chromosome dynamics to enable reduction of accumulating DNA damage in single neurons

Abstract

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